##gff-version 3
##sequence-region BBa_K1362141 1 1583
BBa_K1362141	.	tag	39	59	.	+	0	ID=BBa_K1362431;Name=BBa_K1362431
BBa_K1362141	.	sequence_feature	57	59	.	+	0	ID=annotation2400586;Name=Gly;Parent=BBa_K1362431
BBa_K1362141	.	tag	39	56	.	+	0	ID=annotation2400585;Name=His6;Parent=BBa_K1362431
BBa_K1362141	.	polypeptide_domain	1578	1581	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362141	.	stop_codon	1578	1581	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362141	.	polypeptide_domain	66	490	.	+	0	ID=BBa_K1362409;Name=BBa_K1362409
BBa_K1362141	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362141	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362141	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362141	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362141	.	polypeptide_domain	64	65	.	+	0	ID=BBa_K1362467;Name=BBa_K1362467
BBa_K1362141	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362141	.	engineered_region	495	1577	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362141	.	sequence_feature	1571	1577	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362141	.	engineered_region	502	1570	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362141	.	terminator	1442	1521	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362141	.	stem_loop	1453	1496	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362141	.	engineered_region	1442	1521	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362141	.	terminator	1530	1570	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362141	.	polyA_site	1557	1570	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362141	.	stop_codon	1563	1563	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362141	.	stem_loop	1537	1556	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362141	.	engineered_region	1530	1570	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362141	.	CDS	728	1408	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362141	.	CDS	728	1402	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362141	.	engineered_tag	1409	1433	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362141	.	promoter	502	701	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362141	.	non_covalent_binding_site	667	701	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362141	.	non_covalent_binding_site	590	627	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362141	.	promoter	638	643	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362141	.	start_codon	674	674	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362141	.	engineered_region	502	701	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362141	.	CDS	502	589	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362141	.	promoter	662	667	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362141	.	ribosome_entry_site	710	721	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362141	.	conserved_region	714	717	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362141	.	sequence_feature	495	501	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362141	.	polypeptide_domain	491	494	.	+	0	ID=BBa_K1362419;Name=BBa_K1362419
BBa_K1362141	.	polypeptide_domain	60	63	.	+	0	ID=BBa_K1362418;Name=BBa_K1362418
BBa_K1362141	.	sequence_feature	1582	1583	.	+	0	ID=BBa_K1362465;Name=BBa_K1362465
>BBa_K1362141
aataattttgtttaactttaagaaggagatactagatgcatcatcaccatcaccacggtggtggtagcacaggaaaaag
agtttctattaaagatttgttagatgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctagaatca
gctaaagttagtcgtgtattttgtactggcaaaaagctagtttatattctaaaaactcgactaggtagaactatcaagg
caacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctct
accccgtaaactagaaagctcctctttacaattgtcaccagaaatagaaaagttgtctcagagtgatatttactgggac
tccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggaccacataactttgtcgcca
atgacatcattgtccacaacagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccc
caggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaa
agaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttcc
gttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaag
ttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaa
acacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa
gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggta
ccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgga
agacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacc
acctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacg
aagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgcta
gtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcataa
taa