LOCUS       BBa_K1362143            1582 bp    DNA     linear       25-Nov-2024
DEFINITION  RBS + SspDnaB C-intein RFC[105] non-splicing control construct 
            (with His6)
ACCESSION   BBa_K1362143
VERSION     BBa_K1362143.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..56
                     /label=His6
     misc_feature    57..59
                     /label=Gly
     misc_feature    39..59
                     /label=BBa_K1362431
     misc_feature    60..63
                     /label=BBa_K1362418
     misc_feature    64..488
                     /label=BBa_K1362409
     misc_feature    489..492
                     /label=BBa_K1362420
     misc_feature    493..499
                     /label=BBa_K1362423
     CDS             500..587
                     /label=end of LacI coding region (inactive)
     misc_feature    500..699
                     /label=BBa_R0010
     misc_feature    588..625
                     /label=CAP binding site
     promoter        636..641
                     /label=-35
     promoter        660..665
                     /label=-10
     misc_feature    665..699
                     /label=LacI binding site
     misc_feature    672..672
                     /label=start
     promoter        500..699
                     /label=BBa_R0010
     misc_feature    712..715
                     /label=conserved
     RBS             708..719
                     /label=BBa_B0034
     CDS             726..1400
                     /label=mrfp1
     misc_feature    1407..1431
                     /label=Help:Barcodes
     CDS             726..1406
                     /label=BBa_E1010
     stem_loop       1451..1494
                     /label=stem_loop
     misc_feature    1440..1519
                     /label=BBa_B0010
     terminator      1440..1519
                     /label=BBa_B0010
     stem_loop       1535..1554
                     /label=T7 TE
     misc_feature    1561..1561
                     /label=stop
     polyA_site      1555..1568
                     /label=polya
     misc_feature    1528..1568
                     /label=BBa_B0012
     terminator      1528..1568
                     /label=BBa_B0012
     misc_feature    500..1568
                     /label=BBa_J04450
     misc_feature    1569..1575
                     /label=BBa_K1362424
     misc_feature    493..1575
                     /label=BBa_K1362422
     misc_feature    1576..1579
                     /label=stop
     misc_feature    1576..1579
                     /label=BBa_K1362421
     misc_feature    1580..1581
                     /label=stop
     misc_feature    1580..1582
                     /label=BBa_K1362437
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcaccat caccacggtg
       61 gtgagcacag gaaaaagagt ttctattaaa gatttgttag atgaaaaaga ttttgaaata
      121 tgggcaatta atgaacagac gatgaagcta gaatcagcta aagttagtcg tgtattttgt
      181 actggcaaaa agctagttta tattctaaaa actcgactag gtagaactat caaggcaaca
      241 gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct atctttaaaa
      301 gagcatattg ctctaccccg taaactagaa agctcctctt tacaattgtc accagaaata
      361 gaaaagttgt ctcagagtga tatttactgg gactccatcg tttctattac ggagactgga
      421 gtcgaagagg tttttgattt gactgtgcca ggaccacata actttgtcgc caatgacatc
      481 attgtccaag gcagagaccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      541 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      661 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
      721 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      781 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      841 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      901 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      961 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     1021 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     1081 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     1141 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     1201 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     1261 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     1321 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     1381 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     1441 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1501 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1561 cgtttatagg tctcataata aa
//