##gff-version 3
##sequence-region BBa_K1362143 1 1582
BBa_K1362143	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362143	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362143	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362143	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362143	.	polypeptide_domain	60	63	.	+	0	ID=BBa_K1362418;Name=BBa_K1362418
BBa_K1362143	.	polypeptide_domain	1576	1579	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362143	.	stop_codon	1576	1579	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362143	.	tag	39	59	.	+	0	ID=BBa_K1362431;Name=BBa_K1362431
BBa_K1362143	.	sequence_feature	57	59	.	+	0	ID=annotation2400586;Name=Gly;Parent=BBa_K1362431
BBa_K1362143	.	tag	39	56	.	+	0	ID=annotation2400585;Name=His6;Parent=BBa_K1362431
BBa_K1362143	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362143	.	engineered_region	493	1575	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362143	.	sequence_feature	1569	1575	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362143	.	engineered_region	500	1568	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362143	.	terminator	1440	1519	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362143	.	engineered_region	1440	1519	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362143	.	stem_loop	1451	1494	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362143	.	CDS	726	1406	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362143	.	engineered_tag	1407	1431	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362143	.	CDS	726	1400	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362143	.	terminator	1528	1568	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362143	.	stem_loop	1535	1554	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362143	.	stop_codon	1561	1561	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362143	.	engineered_region	1528	1568	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362143	.	polyA_site	1555	1568	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362143	.	ribosome_entry_site	708	719	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362143	.	conserved_region	712	715	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362143	.	promoter	500	699	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362143	.	promoter	660	665	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362143	.	promoter	636	641	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362143	.	start_codon	672	672	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362143	.	non_covalent_binding_site	665	699	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362143	.	CDS	500	587	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362143	.	non_covalent_binding_site	588	625	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362143	.	engineered_region	500	699	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362143	.	sequence_feature	493	499	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362143	.	polypeptide_domain	489	492	.	+	0	ID=BBa_K1362420;Name=BBa_K1362420
BBa_K1362143	.	sequence_feature	1580	1582	.	+	0	ID=BBa_K1362437;Name=BBa_K1362437
BBa_K1362143	.	stop_codon	1580	1581	.	+	0	ID=annotation2402256;Name=stop;Parent=BBa_K1362437
BBa_K1362143	.	polypeptide_domain	64	488	.	+	0	ID=BBa_K1362409;Name=BBa_K1362409
>BBa_K1362143
aataattttgtttaactttaagaaggagatactagatgcatcatcaccatcaccacggtggtgagcacaggaaaaagag
tttctattaaagatttgttagatgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctagaatcagc
taaagttagtcgtgtattttgtactggcaaaaagctagtttatattctaaaaactcgactaggtagaactatcaaggca
acagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctac
cccgtaaactagaaagctcctctttacaattgtcaccagaaatagaaaagttgtctcagagtgatatttactgggactc
catcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggaccacataactttgtcgccaat
gacatcattgtccaaggcagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaag
aggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcataata
aa