##gff-version 3
##sequence-region BBa_K1362161 1 1265
BBa_K1362161	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362161	.	engineered_region	177	1259	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362161	.	sequence_feature	1253	1259	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362161	.	engineered_region	184	1252	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362161	.	ribosome_entry_site	392	403	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362161	.	conserved_region	396	399	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362161	.	terminator	1124	1203	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362161	.	engineered_region	1124	1203	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362161	.	stem_loop	1135	1178	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362161	.	terminator	1212	1252	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362161	.	engineered_region	1212	1252	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362161	.	polyA_site	1239	1252	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362161	.	stem_loop	1219	1238	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362161	.	stop_codon	1245	1245	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362161	.	promoter	184	383	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362161	.	CDS	184	271	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362161	.	engineered_region	184	383	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362161	.	start_codon	356	356	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362161	.	non_covalent_binding_site	272	309	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362161	.	promoter	344	349	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362161	.	non_covalent_binding_site	349	383	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362161	.	promoter	320	325	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362161	.	CDS	410	1090	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362161	.	CDS	410	1084	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362161	.	engineered_tag	1091	1115	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362161	.	sequence_feature	177	183	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362161	.	polypeptide_domain	173	176	.	+	0	ID=BBa_K1362419;Name=BBa_K1362419
BBa_K1362161	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362161	.	sequence_feature	1264	1265	.	+	0	ID=BBa_K1362465;Name=BBa_K1362465
BBa_K1362161	.	polypeptide_domain	1260	1263	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362161	.	stop_codon	1260	1263	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362161	.	polypeptide_domain	60	63	.	+	0	ID=BBa_K1362418;Name=BBa_K1362418
BBa_K1362161	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362161	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362161	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362161	.	polypeptide_domain	66	172	.	+	0	ID=BBa_K1362413;Name=BBa_K1362413
BBa_K1362161	.	polypeptide_domain	64	65	.	+	0	ID=BBa_K1362467;Name=BBa_K1362467
BBa_K1362161	.	tag	39	59	.	+	0	ID=BBa_K1362431;Name=BBa_K1362431
BBa_K1362161	.	tag	39	56	.	+	0	ID=annotation2400585;Name=His6;Parent=BBa_K1362431
BBa_K1362161	.	sequence_feature	57	59	.	+	0	ID=annotation2400586;Name=Gly;Parent=BBa_K1362431
>BBa_K1362161
aataattttgtttaactttaagaaggagatactagatgcatcatcaccatcaccacggtggtggtatgatgctgaaaaa
aatcctgaaaatcgaagagctggatgaacgtgaactgatcgatattgaggtgtccggtaaccacctgttttacgctaac
gatattctgacccacaacagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaag
aggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcataataa
a