LOCUS BBa_K1362166 2481 bp DNA linear 29-Apr-2024 DEFINITION RBS + Gp41-1 N-intein RFC[105] assembly construct with additional C-teminal insertion site ACCESSION BBa_K1362166 VERSION BBa_K1362166.1 FEATURES Location/Qualifiers RBS 11..28 /label=t7 RBS misc_feature 21..28 /label=Shine-Dalgarno RBS 1..29 /label=BBa_K1362090 misc_feature 30..35 /label=BBa_G0000 misc_feature 36..38 /label=BBa_K1362414 misc_feature 39..45 /label=BBa_K1362423 CDS 46..133 /label=end of LacI coding region (inactive) misc_feature 46..245 /label=BBa_R0010 misc_feature 134..171 /label=CAP binding site promoter 182..187 /label=-35 promoter 206..211 /label=-10 misc_feature 211..245 /label=LacI binding site misc_feature 218..218 /label=start promoter 46..245 /label=BBa_R0010 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 272..946 /label=mrfp1 misc_feature 953..977 /label=Help:Barcodes CDS 272..952 /label=BBa_E1010 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 46..1114 /label=BBa_J04450 misc_feature 1115..1121 /label=BBa_K1362424 misc_feature 39..1121 /label=BBa_K1362422 misc_feature 1122..1125 /label=BBa_K1362415 misc_feature 1126..1385 /label=BBa_K1362412 misc_feature 1388..1391 /label=RFC[???] C misc_feature 1386..1391 /label=GlyGly misc_feature 1386..1391 /label=BBa_K1362417 misc_feature 1392..1398 /label=BBa_K1362423 CDS 1399..1486 /label=end of LacI coding region (inactive) misc_feature 1399..1598 /label=BBa_R0010 misc_feature 1487..1524 /label=CAP binding site promoter 1535..1540 /label=-35 promoter 1559..1564 /label=-10 misc_feature 1564..1598 /label=LacI binding site misc_feature 1571..1571 /label=start promoter 1399..1598 /label=BBa_R0010 misc_feature 1611..1614 /label=conserved RBS 1607..1618 /label=BBa_B0034 CDS 1625..2299 /label=mrfp1 misc_feature 2306..2330 /label=Help:Barcodes CDS 1625..2305 /label=BBa_E1010 stem_loop 2350..2393 /label=stem_loop misc_feature 2339..2418 /label=BBa_B0010 terminator 2339..2418 /label=BBa_B0010 stem_loop 2434..2453 /label=T7 TE misc_feature 2460..2460 /label=stop polyA_site 2454..2467 /label=polya misc_feature 2427..2467 /label=BBa_B0012 terminator 2427..2467 /label=BBa_B0012 misc_feature 1399..2467 /label=BBa_J04450 misc_feature 2468..2474 /label=BBa_K1362424 misc_feature 1392..2474 /label=BBa_K1362422 misc_feature 2475..2478 /label=stop misc_feature 2475..2478 /label=BBa_K1362421 misc_feature 2479..2480 /label=stop misc_feature 2479..2481 /label=BBa_K1362437 ORIGIN 1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc 61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc atgcttggat ctgaaaaccc 1141 aggttcagac cccgcagggt atgaaggaaa tttccaacat ccaggtcggt gatctggtac 1201 tgagcaacac gggttacaac gaagttctga acgtcttccc gaaatctaaa aaaaagtctt 1261 acaaaatcac cctggaagat ggcaaggaaa tcatctgttc cgaagaacac ctgtttccga 1321 cgcagactgg tgaaatgaac atctccggtg gtctgaaaga aggtatgtgt ctgtatgtta 1381 aagaaggtgg tagagaccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1441 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1501 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 1561 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata 1621 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga 1681 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga 1741 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga 1801 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat 1861 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt 1921 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat 1981 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa 2041 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg 2101 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac 2161 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact 2221 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg 2281 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc 2341 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2401 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2461 gtttataggt ctcataataa a //