##gff-version 3
##sequence-region BBa_K1362166 1 2481
BBa_K1362166	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362166	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362166	.	engineered_region	1392	2474	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362166	.	engineered_region	1399	2467	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362166	.	ribosome_entry_site	1607	1618	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362166	.	conserved_region	1611	1614	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362166	.	terminator	2339	2418	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362166	.	engineered_region	2339	2418	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362166	.	stem_loop	2350	2393	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362166	.	promoter	1399	1598	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362166	.	non_covalent_binding_site	1487	1524	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362166	.	promoter	1535	1540	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362166	.	start_codon	1571	1571	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362166	.	CDS	1399	1486	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362166	.	promoter	1559	1564	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362166	.	non_covalent_binding_site	1564	1598	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362166	.	engineered_region	1399	1598	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362166	.	CDS	1625	2305	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362166	.	engineered_tag	2306	2330	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362166	.	CDS	1625	2299	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362166	.	terminator	2427	2467	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362166	.	polyA_site	2454	2467	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362166	.	engineered_region	2427	2467	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362166	.	stem_loop	2434	2453	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362166	.	stop_codon	2460	2460	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362166	.	sequence_feature	2468	2474	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362166	.	sequence_feature	1392	1398	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362166	.	polypeptide_domain	1386	1391	.	+	0	ID=BBa_K1362417;Name=BBa_K1362417
BBa_K1362166	.	conserved_region	1388	1391	.	+	0	ID=annotation2405925;Name=RFC[???] C;Parent=BBa_K1362417
BBa_K1362166	.	null	1386	1391	.	+	0	ID=annotation2405926;Name=GlyGly;Parent=BBa_K1362417
BBa_K1362166	.	sequence_feature	2479	2481	.	+	0	ID=BBa_K1362437;Name=BBa_K1362437
BBa_K1362166	.	stop_codon	2479	2480	.	+	0	ID=annotation2402256;Name=stop;Parent=BBa_K1362437
BBa_K1362166	.	polypeptide_domain	1122	1125	.	+	0	ID=BBa_K1362415;Name=BBa_K1362415
BBa_K1362166	.	polypeptide_domain	2475	2478	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362166	.	stop_codon	2475	2478	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362166	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362166	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362166	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362166	.	polypeptide_domain	1126	1385	.	+	0	ID=BBa_K1362412;Name=BBa_K1362412
BBa_K1362166	.	engineered_region	39	1121	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362166	.	engineered_region	46	1114	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362166	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362166	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362166	.	terminator	986	1065	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362166	.	engineered_region	986	1065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362166	.	stem_loop	997	1040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362166	.	promoter	46	245	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362166	.	non_covalent_binding_site	134	171	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362166	.	promoter	182	187	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362166	.	start_codon	218	218	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362166	.	CDS	46	133	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362166	.	promoter	206	211	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362166	.	non_covalent_binding_site	211	245	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362166	.	engineered_region	46	245	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362166	.	CDS	272	952	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362166	.	engineered_tag	953	977	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362166	.	CDS	272	946	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362166	.	terminator	1074	1114	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362166	.	polyA_site	1101	1114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362166	.	engineered_region	1074	1114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362166	.	stem_loop	1081	1100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362166	.	stop_codon	1107	1107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362166	.	sequence_feature	1115	1121	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362166	.	sequence_feature	39	45	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
>BBa_K1362166
aataattttgtttaactttaagaaggagatactagatgagagacccaatacgcaaaccgcctctccccgcgcgttggcc
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaa
gttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtaccc
agaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacgg
ttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaa
cgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctaca
aagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccac
cgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactac
gacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactgg
acatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta
ataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttataggtctcatgcttggatctgaaaacccaggttcagaccccgcagggtatgaaggaaatttccaacatccagg
tcggtgatctggtactgagcaacacgggttacaacgaagttctgaacgtcttcccgaaatctaaaaaaaagtcttacaa
aatcaccctggaagatggcaaggaaatcatctgttccgaagaacacctgtttccgacgcagactggtgaaatgaacatc
tccggtggtctgaaagaaggtatgtgtctgtatgttaaagaaggtggtagagacccaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagc
ggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcat
gcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtac
gaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagt
tccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggttt
caaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgag
ttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggg
aagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacgg
tggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgac
atcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcca
ccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttataggtctcataataaa