LOCUS BBa_K1362167 2327 bp DNA linear 29-Nov-2024 DEFINITION RBS + Gp41-1 C-intein RFC[105] assembly construct with additional N-teminal insertion site ACCESSION BBa_K1362167 VERSION BBa_K1362167.1 FEATURES Location/Qualifiers RBS 11..28 /label=t7 RBS misc_feature 21..28 /label=Shine-Dalgarno RBS 1..29 /label=BBa_K1362090 misc_feature 30..35 /label=BBa_G0000 misc_feature 36..38 /label=BBa_K1362414 misc_feature 39..45 /label=BBa_K1362423 CDS 46..133 /label=end of LacI coding region (inactive) misc_feature 46..245 /label=BBa_R0010 misc_feature 134..171 /label=CAP binding site promoter 182..187 /label=-35 promoter 206..211 /label=-10 misc_feature 211..245 /label=LacI binding site misc_feature 218..218 /label=start promoter 46..245 /label=BBa_R0010 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 272..946 /label=mrfp1 misc_feature 953..977 /label=Help:Barcodes CDS 272..952 /label=BBa_E1010 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 46..1114 /label=BBa_J04450 misc_feature 1115..1121 /label=BBa_K1362424 misc_feature 39..1121 /label=BBa_K1362422 misc_feature 1122..1125 /label=BBa_K1362418 misc_feature 1126..1127 /label=BBa_K1362467 misc_feature 1128..1234 /label=BBa_K1362413 misc_feature 1235..1238 /label=BBa_K1362419 misc_feature 1239..1245 /label=BBa_K1362423 CDS 1246..1333 /label=end of LacI coding region (inactive) misc_feature 1246..1445 /label=BBa_R0010 misc_feature 1334..1371 /label=CAP binding site promoter 1382..1387 /label=-35 promoter 1406..1411 /label=-10 misc_feature 1411..1445 /label=LacI binding site misc_feature 1418..1418 /label=start promoter 1246..1445 /label=BBa_R0010 misc_feature 1458..1461 /label=conserved RBS 1454..1465 /label=BBa_B0034 CDS 1472..2146 /label=mrfp1 misc_feature 2153..2177 /label=Help:Barcodes CDS 1472..2152 /label=BBa_E1010 stem_loop 2197..2240 /label=stem_loop misc_feature 2186..2265 /label=BBa_B0010 terminator 2186..2265 /label=BBa_B0010 stem_loop 2281..2300 /label=T7 TE misc_feature 2307..2307 /label=stop polyA_site 2301..2314 /label=polya misc_feature 2274..2314 /label=BBa_B0012 terminator 2274..2314 /label=BBa_B0012 misc_feature 1246..2314 /label=BBa_J04450 misc_feature 2315..2321 /label=BBa_K1362424 misc_feature 1239..2321 /label=BBa_K1362422 misc_feature 2322..2325 /label=stop misc_feature 2322..2325 /label=BBa_K1362421 misc_feature 2326..2327 /label=BBa_K1362465 ORIGIN 1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc 61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc aggtggtatg atgctgaaaa 1141 aaatcctgaa aatcgaagag ctggatgaac gtgaactgat cgatattgag gtgtccggta 1201 accacctgtt ttacgctaac gatattctga cccacaacag agacccaata cgcaaaccgc 1261 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1321 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 1381 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 1441 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 1501 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 1561 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 1621 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 1681 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 1741 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 1801 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 1861 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 1921 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 1981 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 2041 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 2101 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 2161 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2221 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2281 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc ataataa //