LOCUS       BBa_K1362173             575 bp    DNA     linear       22-Nov-2024
DEFINITION  Split sfGFP reconstitution N-terminal splicing half
ACCESSION   BBa_K1362173
VERSION     BBa_K1362173.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     CDS             57..245
                     /label=sfGFP(2-64)
     misc_feature    36..38
                     /label=start
     misc_feature    39..56
                     /label=His6
     misc_feature    36..245
                     /label=BBa_K1362471
     misc_feature    246..249
                     /label=BBa_K1362475
     misc_feature    250..551
                     /label=BBa_K1362400
     misc_feature    552..569
                     /label=PolyHis
     misc_feature    552..569
                     /label=BBa_J18909
     misc_feature    570..574
                     /label=stop
     misc_feature    570..575
                     /label=BBa_J70594
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca ccaccaccac caccaccgta
       61 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      121 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      181 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      241 cgctgtgttt aagctatgaa acggaaatat tgacagtaga atatggatta ttaccgattg
      301 gtaaaattgt agaaaagcgc atcgaatgta ctgtttatag cgttgataat aatggaaata
      361 tttatacaca acctgtagca caatggcacg atcgcggaga acaagaggtg tttgagtatt
      421 gtttggaaga tggttcattg attcgggcaa caaaagacca taagtttatg actgttgatg
      481 gtcaaatgtt gccaattgat gaaatatttg aacgtgaatt ggatttgatg cgggttgata
      541 atttgccgaa tcatcatcat catcatcatt aataa
//