##gff-version 3
##sequence-region BBa_K1362174 1 575
BBa_K1362174	.	polypeptide_domain	246	249	.	+	0	ID=BBa_K1362478;Name=BBa_K1362478
BBa_K1362174	.	point_mutation	246	246	.	+	0	ID=annotation2414466;Name=Cys+1Gly;Parent=BBa_K1362478
BBa_K1362174	.	polypeptide_domain	36	245	.	+	0	ID=BBa_K1362471;Name=BBa_K1362471
BBa_K1362174	.	tag	39	56	.	+	0	ID=annotation2414299;Name=His6;Parent=BBa_K1362471
BBa_K1362174	.	CDS	57	245	.	+	0	ID=annotation2414077;Name=sfGFP(2-64);Parent=BBa_K1362471
BBa_K1362174	.	start_codon	36	38	.	+	0	ID=annotation2414078;Name=start;Parent=BBa_K1362471
BBa_K1362174	.	polypeptide_domain	250	551	.	+	0	ID=BBa_K1362400;Name=BBa_K1362400
BBa_K1362174	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362174	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362174	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362174	.	polypeptide_domain	570	575	.	+	0	ID=BBa_J70594;Name=BBa_J70594
BBa_K1362174	.	stop_codon	570	574	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362174	.	tag	552	569	.	+	0	ID=BBa_J18909;Name=BBa_J18909
BBa_K1362174	.	tag	552	569	.	+	0	ID=annotation2065463;Name=PolyHis;Parent=BBa_J18909
BBa_K1362174	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
>BBa_K1362174
aataattttgtttaactttaagaaggagatactagatgcaccaccaccaccaccaccgtaaaggcgaagagctgttcac
tggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggt
gacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaa
cgacgctgggtttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaa
gcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgc
ggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactg
ttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaatca
tcatcatcatcatcattaataa