##gff-version 3
##sequence-region BBa_K1362204 1 1160
BBa_K1362204	.	engineered_region	67	1135	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1362204	.	terminator	1007	1086	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362204	.	stem_loop	1018	1061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362204	.	engineered_region	1007	1086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362204	.	terminator	1095	1135	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362204	.	stop_codon	1128	1128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362204	.	polyA_site	1122	1135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362204	.	stem_loop	1102	1121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362204	.	engineered_region	1095	1135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362204	.	CDS	293	973	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362204	.	CDS	293	967	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362204	.	engineered_tag	974	998	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362204	.	promoter	67	266	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362204	.	non_covalent_binding_site	155	192	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362204	.	non_covalent_binding_site	232	266	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362204	.	promoter	203	208	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362204	.	start_codon	239	239	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362204	.	engineered_region	67	266	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362204	.	CDS	67	154	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362204	.	promoter	227	232	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362204	.	ribosome_entry_site	275	286	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362204	.	conserved_region	279	282	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362204	.	polypeptide_domain	40	60	.	+	0	ID=BBa_K1362453;Name=BBa_K1362453
BBa_K1362204	.	tag	13	30	.	+	0	ID=BBa_K1362458;Name=BBa_K1362458
BBa_K1362204	.	polypeptide_domain	1143	1154	.	+	0	ID=BBa_K1362459;Name=BBa_K1362459
BBa_K1362204	.	polypeptide_domain	1155	1160	.	+	0	ID=BBa_J70594;Name=BBa_J70594
BBa_K1362204	.	stop_codon	1155	1159	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362204	.	sequence_feature	1136	1142	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424
BBa_K1362204	.	polypeptide_domain	31	39	.	+	0	ID=BBa_K1362449;Name=BBa_K1362449
BBa_K1362204	.	polypeptide_domain	1	12	.	+	0	ID=BBa_K1362445;Name=BBa_K1362445
BBa_K1362204	.	sequence_feature	61	66	.	+	0	ID=BBa_K1362447;Name=BBa_K1362447
>BBa_K1362204
atgggcagtagtcatcatcatcatcatcacagcagcggcgaaaacctgtacttccagggtagagaccaatacgcaaacc
gcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgc
aacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtg
gaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttataggtctcacttccggaaacctaataa