BBa_K1362473
1
Stop
C terminal Stop insert for RFC[???] N intein constructs with two insertion sites
2014-10-08T11:00:00Z
2015-05-08T01:10:06Z
This Sequence is used to close the N-terminal insertion site of an RFC[???] N-intein assembly construct with two insertions sites by introducing an early stop codon resulting an an RFC[???] N-intein assembly construct with only one insertion site.
false
false
_1738_
0
22830
9
Not in stock
false
As a sequence of length 0 could not be cloned as an oligo into an RFC[???] the sequence starts with two stop codons to end the future POI-C intein cds as soon as possible. For better oligo annealing and thus ligation the sequence was extended for 6 nucleotides.
false
Jan Gleixner
annotation2411982
1
stop
range2411982
1
1
6
BBa_K1362473_sequence
1
tagtagggtggc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z