BBa_K1362450 1 mDNMT1(731 Murine DNMT1, residues 731-1602 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z Mus musculus; codon-optimized for E. coli This part codes for the amino acides 731 through 1602 of DNA (cytosine-5)-methyltransferase 1 (DNMT1) from ''Mus musculus'', containing the BAH1, BAH2, and methyltransferase domains [[{{PAGENAME}}:Design#References|[1]]]. It is codon-optimized for ''E. coli''. false false _1738_ 0 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362429 1 Smt3 ubiquitin-like protein Smt3 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z S. cerevisiae keine Ahnung false false _1738_ 0 22920 9 Not in stock false ??? false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2407301 1 Smt3 range2407301 1 1 294 BBa_K1362456 1 Thrombin c Thrombin recognition/cleavage site 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z This part codes for the amino acid sequence LVPRGS. This site is recognized by Thrombin, which will cleave the Arg-Gly peptide bond. false false _1738_ 0 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_J70594 1 BBa_J70594 RFC12 TAATAA Tail Domain 2010-06-17T11:00:00Z 2015-05-08T01:08:25Z Common Knowledge A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag. false true _41_ 0 6384 41 Not in stock false Made with synthetic oligos: 5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3' 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3' Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. false Joseph Lynch annotation2071257 1 stop range2071257 1 1 5 BBa_K1362449 1 SSG SSG linker 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z Sequence excerpt from the pET-28a-c(+) series of vectors. This part codes for the amino acid sequence SSG. false false _1738_ 0 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362448 1 HMASM HMASM linker 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z Sequence excerpt from the pET-28a-c(+) series of vectors. This part codes for the amino acid sequence HMASM. false false _1738_ 0 22951 9 Not in stock false This is an excerpt from the pET-28a-c(+) series of vectors. The MASM is the beginning of the T7 tag sequence. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362446 1 MGSS Start codon and GSS spacer 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z designed manually This part codes for the protein sequence MGSS. false false _1738_ 0 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362500 1 Smt3-mDNMT Smt3-Murine DNMT1(731-1602) fusion protein (with His6) 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z * Smt3: ''Saccharomyces cerevisiae'' * DNMT1: ''Mus musculus'' TODO false false _1738_ 0 22951 9 In stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha component2410950 1 BBa_K1362449 component2410952 1 BBa_K1362448 component2410949 1 BBa_K1362458 component2410957 1 BBa_J70594 component2410948 1 BBa_K1362446 component2410951 1 BBa_K1362456 component2410955 1 BBa_K1362450 component2410954 1 BBa_K1362429 annotation2410955 1 BBa_K1362450 range2410955 1 367 2982 annotation2410949 1 BBa_K1362458 range2410949 1 13 30 annotation2410950 1 BBa_K1362449 range2410950 1 31 39 annotation2410952 1 BBa_K1362448 range2410952 1 58 72 annotation2410957 1 BBa_J70594 range2410957 1 2983 2988 annotation2410951 1 BBa_K1362456 range2410951 1 40 57 annotation2410948 1 BBa_K1362446 range2410948 1 1 12 annotation2410954 1 BBa_K1362429 range2410954 1 73 366 BBa_K1362458 1 His6 Hexahistidine tag 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z This part codes for a hexahistidine tag, which can be used in protein purification by affinity chromatography. false false _1738_ 0 22951 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_J70594_sequence 1 taataa BBa_K1362448_sequence 1 catatggctagcatg BBa_K1362450_sequence 1 aaagatcgcattagctggctgggtcagccgatgaaaattgaagaaaatcgcacctattatcagaaagtgagcattgatgaagaaatgctggaagttggtgattgtgttagcgttattccggatgatagcagcaaaccgctgtatctggcacgtgttaccgcactgtgggaagataaaaatggccagatgatgtttcatgcccattggttttgtgcaggcaccgataccgttctgggtgcaaccagcgatccgctggaactgtttctggttggtgaatgcgaaaatatgcagctgagctatattcatagcaaagtgaaagtgatttataaagcaccgagcgaaaattgggcaatggaaggtggcaccgatccggaaaccaccctgcctggtgcagaagatggtaaaacctatttttttcagctgtggtataatcaggaatatgcccgttttgaaagccctccgaaaacccagccgaccgaagataataaacataaattttgcctgagctgtattcgtctggcagaactgcgtcagaaagaaatgccgaaagtgctggaacaaattgaagaagttgacggtcgcgtttattgtagcagcattaccaaaaatggtgtggtttatcgtctgggtgatagcgtttatctgcctccggaagcatttacctttaatatcaaagtggcctctccggttaaacgtccgaaaaaagatccggttaatgaaaccctgtatccggaacattatcgcaaatatagcgattatattaaaggcagcaatctggatgcaccggaaccgtatcgtattggtcgcattaaagaaattcattgcggtaaaaaaaaaggcaaagtgaatgaagccgatattaaactgcgcctgtataaattttatcgtccggaaaatacccatcgtagctataatggtagctatcataccgatattaatatgctgtattggtccgatgaagaagccgttgttaatttttctgatgttcagggtcgttgtaccgttgaatatggtgaagatctgctggaaagcattcaggattatagccagggtggtccggatcgtttttattttctggaagcctataatagcaaaaccaaaaattttgaagatcctccgaatcatgcacgttctccgggtaataaaggtaaaggcaagggtaaagggaaaggtaagggcaaacatcaggttagcgaaccgaaagaaccggaagcagcaattaaactgccgaaactgcgtaccctggatgtttttagcggttgtggtggtctgagcgaaggttttcatcaggcaggtattagcgaaaccctgtgggcaattgaaatgtgggacccggcagcacaggcatttcgtctgaataatccgggtacaaccgtttttaccgaagattgtaatgtgctgctgaaactggttatggcaggcgaagttaccaatagcctgggtcagcgtctgccgcagaaaggtgatgttgaaatgctgtgtggtggtccgccgtgtcagggttttagcggtatgaatcgctttaatagccgcacctatagcaaattcaaaaatagcctggtggtgagctttctgagctattgcgattattatcgtccgcgtttttttctgctggaaaatgtgcgtaattttgtgagctatcgtcgtagcatggttctgaaactgaccctgcgttgtctggttcgtatgggttatcagtgtacctttggtgttctgcaagcaggtcagtatggtgttgcacagacccgtcgtcgtgcaattattctggcagcagcaccgggtgaaaagctgccgctgtttccggaaccgctgcatgtgtttgcaccgcgtgcatgtcagctgagcgttgttgtggatgataaaaaatttgtgagcaatattacccgtctgagcagcggtccgtttcgtaccattaccgttcgtgataccatgagcgatctgccggaaattcagaatggtgcaagcaatagcgaaattccgtataatggtgaaccgctgagctggtttcagcgtcagctgcgtggtagccattatcagccgattctgcgtgatcatatttgtaaagatatgtctccgctggttgcagcacgtatgcgtcatattccgctgttcccgggtagcgattggcgtgatctgccgaatattcaggttcgcctgggtgatggtgttattgcacataaactgcaatatacctttcatgatgtgaaaaatggctatagcagcacaggtgcactgcgtggtgtttgtagctgtgcagaaggtaaagcctgcgatccggaaagccgtcagtttagcaccctgattccgtggtgtctgccgcataccggtaatcgtcataatcattgggcaggtctgtatggccgtctggaatgggatggtttttttagcaccaccgttaccaatccggaaccgatgggtaaacagggtcgtgttctgcatccggaacagcatcgtgttgttagcgttcgtgaatgtgcacgtagccagggttttccggatagctatcgcttttttggcaatattctggatcgtcatcgtcaggttggtaatgcagttccgcctccgctggcaaaagcaattggcctggaaattaaactgtgtctgctgagcagcgca BBa_K1362456_sequence 1 ctggtgccgcgcggcagc BBa_K1362500_sequence 1 atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgtcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaaagacagggtaaggaaatggactccttaagattcttgtacgacggtattagaatccaagctgatcagacccctgaagatttggacatggaggataacgatattattgaggctcacagagaacagattggtggatccaaagatcgcattagctggctgggtcagccgatgaaaattgaagaaaatcgcacctattatcagaaagtgagcattgatgaagaaatgctggaagttggtgattgtgttagcgttattccggatgatagcagcaaaccgctgtatctggcacgtgttaccgcactgtgggaagataaaaatggccagatgatgtttcatgcccattggttttgtgcaggcaccgataccgttctgggtgcaaccagcgatccgctggaactgtttctggttggtgaatgcgaaaatatgcagctgagctatattcatagcaaagtgaaagtgatttataaagcaccgagcgaaaattgggcaatggaaggtggcaccgatccggaaaccaccctgcctggtgcagaagatggtaaaacctatttttttcagctgtggtataatcaggaatatgcccgttttgaaagccctccgaaaacccagccgaccgaagataataaacataaattttgcctgagctgtattcgtctggcagaactgcgtcagaaagaaatgccgaaagtgctggaacaaattgaagaagttgacggtcgcgtttattgtagcagcattaccaaaaatggtgtggtttatcgtctgggtgatagcgtttatctgcctccggaagcatttacctttaatatcaaagtggcctctccggttaaacgtccgaaaaaagatccggttaatgaaaccctgtatccggaacattatcgcaaatatagcgattatattaaaggcagcaatctggatgcaccggaaccgtatcgtattggtcgcattaaagaaattcattgcggtaaaaaaaaaggcaaagtgaatgaagccgatattaaactgcgcctgtataaattttatcgtccggaaaatacccatcgtagctataatggtagctatcataccgatattaatatgctgtattggtccgatgaagaagccgttgttaatttttctgatgttcagggtcgttgtaccgttgaatatggtgaagatctgctggaaagcattcaggattatagccagggtggtccggatcgtttttattttctggaagcctataatagcaaaaccaaaaattttgaagatcctccgaatcatgcacgttctccgggtaataaaggtaaaggcaagggtaaagggaaaggtaagggcaaacatcaggttagcgaaccgaaagaaccggaagcagcaattaaactgccgaaactgcgtaccctggatgtttttagcggttgtggtggtctgagcgaaggttttcatcaggcaggtattagcgaaaccctgtgggcaattgaaatgtgggacccggcagcacaggcatttcgtctgaataatccgggtacaaccgtttttaccgaagattgtaatgtgctgctgaaactggttatggcaggcgaagttaccaatagcctgggtcagcgtctgccgcagaaaggtgatgttgaaatgctgtgtggtggtccgccgtgtcagggttttagcggtatgaatcgctttaatagccgcacctatagcaaattcaaaaatagcctggtggtgagctttctgagctattgcgattattatcgtccgcgtttttttctgctggaaaatgtgcgtaattttgtgagctatcgtcgtagcatggttctgaaactgaccctgcgttgtctggttcgtatgggttatcagtgtacctttggtgttctgcaagcaggtcagtatggtgttgcacagacccgtcgtcgtgcaattattctggcagcagcaccgggtgaaaagctgccgctgtttccggaaccgctgcatgtgtttgcaccgcgtgcatgtcagctgagcgttgttgtggatgataaaaaatttgtgagcaatattacccgtctgagcagcggtccgtttcgtaccattaccgttcgtgataccatgagcgatctgccggaaattcagaatggtgcaagcaatagcgaaattccgtataatggtgaaccgctgagctggtttcagcgtcagctgcgtggtagccattatcagccgattctgcgtgatcatatttgtaaagatatgtctccgctggttgcagcacgtatgcgtcatattccgctgttcccgggtagcgattggcgtgatctgccgaatattcaggttcgcctgggtgatggtgttattgcacataaactgcaatatacctttcatgatgtgaaaaatggctatagcagcacaggtgcactgcgtggtgtttgtagctgtgcagaaggtaaagcctgcgatccggaaagccgtcagtttagcaccctgattccgtggtgtctgccgcataccggtaatcgtcataatcattgggcaggtctgtatggccgtctggaatgggatggtttttttagcaccaccgttaccaatccggaaccgatgggtaaacagggtcgtgttctgcatccggaacagcatcgtgttgttagcgttcgtgaatgtgcacgtagccagggttttccggatagctatcgcttttttggcaatattctggatcgtcatcgtcaggttggtaatgcagttccgcctccgctggcaaaagcaattggcctggaaattaaactgtgtctgctgagcagcgcataataa BBa_K1362449_sequence 1 agcagcggc BBa_K1362458_sequence 1 catcatcatcatcatcac BBa_K1362446_sequence 1 atgggcagcagc BBa_K1362429_sequence 1 tcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaaagacagggtaaggaaatggactccttaagattcttgtacgacggtattagaatccaagctgatcagacccctgaagatttggacatggaggataacgatattattgaggctcacagagaacagattggtggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z