BBa_K1362616
type: ComponentDefinition
persistent identity: BBa K1362616
display id: BBa_K1362616
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable notes: ???
star: false
creator: Jan Gleixner
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K1362090
sequence annotation: t7 RBS
sequence annotation: Shine-Dalgarno
sequence annotation: BBa_G0000
sequence annotation: Start
sequence annotation: RFC[???] standard overhang A
sequence annotation: BBa_K1362600
sequence annotation: BBa_K1362614
sequence annotation: BBa_K1362599
sequence annotation: BsaI recognition site
sequence annotation: BBa_R0010
sequence annotation: BBa_J04450
sequence annotation: CAP
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI
sequence annotation: start
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_E1010
sequence annotation: mrfp1
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_K1362598
sequence annotation: RFC[???] standart overhang B (Cys)
sequence annotation: BBa_K1362601
sequence annotation: BBa_K1362698
sequence annotation: BBa_K1362602
sequence annotation: RFC[???] standard overhng E
sequence annotation: BBa_K1362606
sequence annotation: His6
sequence annotation: Stop
sequence annotation: RFC[???] standard overhang O
sequence annotation: BBa_K1362603

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2396142
display id: annotation2396142
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396142/range2396142/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396142/range2396142/1
type: Range
persistent identity: range2396142
display id: range2396142
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1
end: 29
orientation: inline

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2396140
display id: annotation2396140
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396140/range2396140/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396140/range2396140/1
type: Range
persistent identity: range2396140
display id: range2396140
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 11
end: 28
orientation: inline

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2396141
display id: annotation2396141
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396141/range2396141/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396141/range2396141/1
type: Range
persistent identity: range2396141
display id: range2396141
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 21
end: 28
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2396143
display id: annotation2396143
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396143/range2396143/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396143/range2396143/1
type: Range
persistent identity: range2396143
display id: range2396143
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 30
end: 35
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2396144
display id: annotation2396144
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396144/range2396144/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396144/range2396144/1
type: Range
persistent identity: range2396144
display id: range2396144
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 36
end: 38
orientation: inline

RFC[???] standard overhang A
type: SequenceAnnotation
persistent identity: annotation2396145
display id: annotation2396145
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396145/range2396145/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396145/range2396145/1
type: Range
persistent identity: range2396145
display id: range2396145
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 36
end: 38
orientation: inline

BBa_K1362600
type: SequenceAnnotation
persistent identity: annotation2396146
display id: annotation2396146
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396146/range2396146/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396146/range2396146/1
type: Range
persistent identity: range2396146
display id: range2396146
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 36
end: 38
orientation: inline

BBa_K1362614
type: SequenceAnnotation
persistent identity: annotation2396169
display id: annotation2396169
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396169/range2396169/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396169/range2396169/1
type: Range
persistent identity: range2396169
display id: range2396169
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 39
end: 1121
orientation: inline

BBa_K1362599
type: SequenceAnnotation
persistent identity: annotation2396147
display id: annotation2396147
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396147/range2396147/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396147/range2396147/1
type: Range
persistent identity: range2396147
display id: range2396147
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 39
end: 45
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2396148
display id: annotation2396148
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396148/range2396148/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396148/range2396148/1
type: Range
persistent identity: range2396148
display id: range2396148
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 40
end: 45
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2396149
display id: annotation2396149
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396149/range2396149/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2396149
display id: range2396149
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 46
end: 245
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2396150
display id: annotation2396150
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396150/range2396150/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396150/range2396150/1
type: Range
persistent identity: range2396150
display id: range2396150
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 46
end: 1114
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2396151
display id: annotation2396151
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396151/range2396151/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396151/range2396151/1
type: Range
persistent identity: range2396151
display id: range2396151
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 134
end: 171
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2396152
display id: annotation2396152
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396152/range2396152/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396152/range2396152/1
type: Range
persistent identity: range2396152
display id: range2396152
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 182
end: 187
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2396153
display id: annotation2396153
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396153/range2396153/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396153/range2396153/1
type: Range
persistent identity: range2396153
display id: range2396153
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 206
end: 211
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2396154
display id: annotation2396154
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396154/range2396154/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396154/range2396154/1
type: Range
persistent identity: range2396154
display id: range2396154
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 211
end: 245
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2396155
display id: annotation2396155
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396155/range2396155/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396155/range2396155/1
type: Range
persistent identity: range2396155
display id: range2396155
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 218
end: 218
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2396156
display id: annotation2396156
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396156/range2396156/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396156/range2396156/1
type: Range
persistent identity: range2396156
display id: range2396156
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 254
end: 265
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2396157
display id: annotation2396157
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396157/range2396157/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396157/range2396157/1
type: Range
persistent identity: range2396157
display id: range2396157
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 258
end: 261
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2396158
display id: annotation2396158
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396158/range2396158/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396158/range2396158/1
type: Range
persistent identity: range2396158
display id: range2396158
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 272
end: 952
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2396159
display id: annotation2396159
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396159/range2396159/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396159/range2396159/1
type: Range
persistent identity: range2396159
display id: range2396159
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 272
end: 946
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2396160
display id: annotation2396160
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396160/range2396160/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396160/range2396160/1
type: Range
persistent identity: range2396160
display id: range2396160
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 953
end: 977
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2396161
display id: annotation2396161
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396161/range2396161/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396161/range2396161/1
type: Range
persistent identity: range2396161
display id: range2396161
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 986
end: 1065
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2396162
display id: annotation2396162
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396162/range2396162/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396162/range2396162/1
type: Range
persistent identity: range2396162
display id: range2396162
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 997
end: 1040
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2396163
display id: annotation2396163
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396163/range2396163/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396163/range2396163/1
type: Range
persistent identity: range2396163
display id: range2396163
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1074
end: 1114
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2396164
display id: annotation2396164
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396164/range2396164/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396164/range2396164/1
type: Range
persistent identity: range2396164
display id: range2396164
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1081
end: 1100
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2396165
display id: annotation2396165
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396165/range2396165/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396165/range2396165/1
type: Range
persistent identity: range2396165
display id: range2396165
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1101
end: 1114
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2396166
display id: annotation2396166
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396166/range2396166/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396166/range2396166/1
type: Range
persistent identity: range2396166
display id: range2396166
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1107
end: 1107
orientation: inline

BBa_K1362598
type: SequenceAnnotation
persistent identity: annotation2396168
display id: annotation2396168
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396168/range2396168/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2396168
display id: range2396168
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1115
end: 1121
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2396167
display id: annotation2396167
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396167/range2396167/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396167/range2396167/1
type: Range
persistent identity: range2396167
display id: range2396167
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1115
end: 1120
orientation: inline

RFC[???] standart overhang B (Cys)
type: SequenceAnnotation
persistent identity: annotation2396170
display id: annotation2396170
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396170/range2396170/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396170/range2396170/1
type: Range
persistent identity: range2396170
display id: range2396170
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1122
end: 1125
orientation: inline

BBa_K1362601
type: SequenceAnnotation
persistent identity: annotation2396171
display id: annotation2396171
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396171/range2396171/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396171/range2396171/1
type: Range
persistent identity: range2396171
display id: range2396171
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1122
end: 1125
orientation: inline

BBa_K1362698
type: SequenceAnnotation
persistent identity: annotation2396172
display id: annotation2396172
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396172/range2396172/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2396172
display id: range2396172
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1126
end: 1427
orientation: inline

BBa_K1362602
type: SequenceAnnotation
persistent identity: annotation2396174
display id: annotation2396174
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396174/range2396174/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396174/range2396174/1
type: Range
persistent identity: range2396174
display id: range2396174
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1428
end: 1433
orientation: inline

RFC[???] standard overhng E
type: SequenceAnnotation
persistent identity: annotation2396173
display id: annotation2396173
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396173/range2396173/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396173/range2396173/1
type: Range
persistent identity: range2396173
display id: range2396173
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1430
end: 1433
orientation: inline

BBa_K1362606
type: SequenceAnnotation
persistent identity: annotation2396177
display id: annotation2396177
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396177/range2396177/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396177/range2396177/1
type: Range
persistent identity: range2396177
display id: range2396177
version: 1
top level: BBa_K1362616
direction: direction/reverse
start: 1434
end: 1457
orientation: inline

His6
type: SequenceAnnotation
persistent identity: annotation2396175
display id: annotation2396175
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396175/range2396175/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396175/range2396175/1
type: Range
persistent identity: range2396175
display id: range2396175
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1437
end: 1454
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2396176
display id: annotation2396176
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396176/range2396176/1
role: feature/stop
role: SO:0000319

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type: Range
persistent identity: range2396176
display id: range2396176
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1455
end: 1457
orientation: inline

RFC[???] standard overhang O
type: SequenceAnnotation
persistent identity: annotation2396178
display id: annotation2396178
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396178/range2396178/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396178/range2396178/1
type: Range
persistent identity: range2396178
display id: range2396178
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1458
end: 1461
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2396179
display id: annotation2396179
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396179/range2396179/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396179/range2396179/1
type: Range
persistent identity: range2396179
display id: range2396179
version: 1
top level: BBa_K1362616
direction: direction/forward
start: 1458
end: 1460
orientation: inline

BBa_K1362603
type: SequenceAnnotation
persistent identity: annotation2396181
display id: annotation2396181
version: 1
top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396181/range2396181/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396181/range2396181/1
type: Range
persistent identity: range2396181
display id: range2396181
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direction: direction/reverse
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Stop
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persistent identity: annotation2396180
display id: annotation2396180
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top level: BBa_K1362616
location: https://synbiohub.org/public/igem/BBa_K1362616/annotation2396180/range2396180/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362616/annotation2396180/range2396180/1
type: Range
persistent identity: range2396180
display id: range2396180
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direction: direction/forward
start: 1461
end: 1463
orientation: inline

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z