##gff-version 3
##sequence-region BBa_K1363006 1 1448
BBa_K1363006	.	engineered_region	380	1448	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1363006	.	CDS	606	1286	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1363006	.	CDS	606	1280	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1363006	.	engineered_tag	1287	1311	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1363006	.	terminator	1408	1448	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1363006	.	polyA_site	1435	1448	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1363006	.	engineered_region	1408	1448	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1363006	.	stem_loop	1415	1434	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1363006	.	stop_codon	1441	1441	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1363006	.	promoter	380	579	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1363006	.	start_codon	552	552	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1363006	.	CDS	380	467	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1363006	.	engineered_region	380	579	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1363006	.	promoter	540	545	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1363006	.	non_covalent_binding_site	545	579	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1363006	.	non_covalent_binding_site	468	505	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1363006	.	promoter	516	521	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1363006	.	ribosome_entry_site	588	599	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1363006	.	conserved_region	592	595	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1363006	.	terminator	1320	1399	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1363006	.	stem_loop	1331	1374	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1363006	.	engineered_region	1320	1399	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1363006	.	oriT	1	371	.	+	0	ID=BBa_J01003;Name=BBa_J01003
>BBa_K1363006
gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggct
tggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgtt
gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgccc
ggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatg
gatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagcaatacgcaaaccgcc
tctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaa
ttgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaa
gagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtc
gtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtc
cccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccg
gaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaag
acggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccat
gggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactg
aaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttaca
aaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcg
tcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata