LOCUS BBa_K1363400 4089 bp DNA linear 15-Dec-2024 DEFINITION R0010-K592016-B0015-K502020 ACCESSION BBa_K1363400 VERSION BBa_K1363400.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1357 /label=YF1 CDS 227..1357 /label=BBa_K592004 misc_feature 1370..1373 /label=conserved RBS 1366..1377 /label=BBa_B0034 CDS 1384..2004 /label=FixJ CDS 1384..2004 /label=BBa_K592005 misc_feature 209..2004 /label=BBa_K592016 stem_loop 2024..2067 /label=stem_loop misc_feature 2013..2092 /label=BBa_B0010 terminator 2013..2092 /label=BBa_B0010 stem_loop 2108..2127 /label=T7 TE misc_feature 2134..2134 /label=stop polyA_site 2128..2141 /label=polya misc_feature 2101..2141 /label=BBa_B0012 terminator 2101..2141 /label=BBa_B0012 terminator 2013..2141 /label=BBa_B0015 promoter 2150..2399 /label=FixK2 promoter promoter 2150..2399 /label=BBa_K592006 misc_feature 2412..2415 /label=conserved RBS 2408..2419 /label=BBa_B0034 CDS 2429..3136 /label=cI lambda misc_feature 3137..3169 /label=LVA misc_feature 3176..3200 /label=Help:Barcodes CDS 2426..3175 /label=BBa_C0051 stem_loop 3220..3263 /label=stem_loop misc_feature 3209..3288 /label=BBa_B0010 terminator 3209..3288 /label=BBa_B0010 stem_loop 3304..3323 /label=T7 TE misc_feature 3330..3330 /label=stop polyA_site 3324..3337 /label=polya misc_feature 3297..3337 /label=BBa_B0012 terminator 3297..3337 /label=BBa_B0012 promoter 3383..3388 /label=-10 promoter 3360..3365 /label=-35 misc_feature 3370..3386 /label=OR1 misc_feature 3346..3362 /label=OR2 misc_feature 3346..3394 /label=BBa_R0051 promoter 3346..3394 /label=BBa_R0051 misc_feature 2408..3394 /label=BBa_Q04510 misc_feature 3407..3410 /label=conserved RBS 3403..3414 /label=BBa_B0034 CDS 3421..4086 /label=amilCP CDS 3421..4089 /label=BBa_K592009 misc_feature 2150..4089 /label=BBa_K592020 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagttttca 241 atcatttggg ataccaggac agctggaagt catcaaaaaa gcacttgatc acgtgcgagt 301 cggtgtggta attacagatc ccgcacttga agataatcct attgtctacg taaatcaagg 361 ctttgttcaa atgaccggct acgagaccga ggaaatttta ggaaagaact gtcgcttctt 421 acaggggaaa cacacagatc cggcagaagt ggacaacatc agaaccgctt tacaaaataa 481 agaaccggtc accgttcaga tccaaaacta caaaaaagac ggaacgatgt tctggaatga 541 attaaatatt gatccaatgg aaatagagga taaaacgtat tttgtcggca ttcagaatga 601 tatcaccgag caccagcaga cccaggcgcg tctccaggaa ctgcaatccg agctcgtcca 661 cgtctccagg ctgagcgcca tgggcgaaat ggcgtccgcg ctcgcgcacg agctcaacca 721 gccgctggcg gcgatcagca actacatgaa gggctcgcgg cggctgcttg ccggcagcag 781 tgatccgaac acaccgaagg tcgaaagcgc cctggaccgc gccgccgagc aggcgctgcg 841 cgccggccag atcatccggc gcctgcgcga cttcgttgcc cgcggcgaat cggagaagcg 901 ggtcgagagt ctctccaagc tgatcgagga ggccggcgcg ctcgggcttg ccggcgcgcg 961 cgagcagaac gtgcagctcc gcttcagtct cgatccgggc gccgatctcg ttctcgccga 1021 ccgggtgcag atccagcagg tcctggtcaa cctgttccgc aacgcgctgg aagcgatggc 1081 tcagtcgcag cgacgcgagc tcgtcgtcac caacaccccc gccgccgacg acatgatcga 1141 ggtcgaagtg tccgacaccg gcagcggttt ccaggacgac gtcattccga acctgtttca 1201 gactttcttc accaccaagg acaccggcat gggcgtggga ctgtccatca gccgctcgat 1261 catcgaagct cacggcgggc gcatgtgggc cgagagcaac gcatcgggcg gggcgacctt 1321 ccgcttcacc ctcccggcag ccgacgagaa ttaataatac tagagaaaga ggagaaatac 1381 tagatgacga ccaagggaca tatctacgtc atcgacgacg acgcggcgat gcgggattcg 1441 ctgaatttcc tgctggattc tgccggcttc ggcgtcacgc tgtttgacga cgcgcaagcc 1501 tttctcgacg ccctgccggg tctctccttc ggctgcgtcg tctccgacgt gcgcatgccg 1561 ggccttgacg gcatcgagct gttgaagcgg atgaaggcgc agcaaagccc ctttccgatc 1621 ctcatcatga ccggtcacgg cgacgtgccg ctcgcggtcg aggcgatgaa gttaggggcg 1681 gtcgactttc tggaaaagcc tttcgaggac gaccgcctca ccgccatgat cgaatcggcg 1741 atccgccagg ccgagccggc cgccaagagc gaggccgtcg cgcaggatat cgccgcccgc 1801 gtcgcctcgt tgagccccag ggagcgccag gtcatggaag ggctgatcgc cggcctttcc 1861 aacaagctga tcgcccgcga gtacgacatc agcccgcgca ccatcgaggt gtatcgggcc 1921 aacgtcatga ccaagatgca ggccaacagc ctttcggagc tggttcgcct cgcgatgcgc 1981 gccggcatgc tcaacgatta ataatactag agccaggcat caaataaaac gaaaggctca 2041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2101 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg ccggagccga 2161 ttatccgcac ccgtccttgg tcttggacac actcgctcgc gatccgaagc cgcctttcaa 2221 agatactccg cagtgaaacg catcttttaa gcgcgacttg tcgccttcgc gcagcagaac 2281 actcgtatgg cactcaatcc atgatcgctt cgttcgctcc gacgcgcgtt gcggggcccc 2341 cctcgaccgg atgacaacac attgcgtcac ctcaactgct gcgcgcgcac tgcgtcacat 2401 actagagaaa gaggagaaat actagatgag cacaaaaaag aaaccattaa cacaagagca 2461 gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt 2521 atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt 2581 taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt 2641 tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt 2701 tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc 2761 agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag 2821 cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac 2881 cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga 2941 gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt 3001 caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc 3061 aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc 3121 tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg 3181 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt 3361 tgactatttt acctctggcg gtgataatgg ttgctactag agaaagagga gaaatactag 3421 atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac ggtcaatgga 3481 cactactttg aggtcgaagg cgatggaaaa ggtaagccct acgaggggga gcagacggta 3541 aagctcactg tcaccaaggg cggacctctg ccatttgctt gggatatttt atcaccacag 3601 tgtcagtacg gaagcatacc attcaccaag taccctgaag acatccctga ctatgtaaag 3661 cagtcattcc cggagggcta tacatgggag aggatcatga actttgaaga tggtgcagtg 3721 tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacca tgtcaagttc 3781 tctggtttga actttcctcc caatggacct gtcatgcaga agaagacaca gggctgggaa 3841 cccaacactg agcgtctctt tgcacgagat ggaatgctgc taggaaacaa ctttatggct 3901 ctgaagttag aaggaggcgg tcactatttg tgtgaattta aaactactta caaggcaaag 3961 aagcctgtga agatgccagg gtatcactat gttgaccgca aactggatgt aaccaatcac 4021 aacaaggatt acacttcggt tgagcagtgt gaaatttcca ttgcacgcaa acctgtggtc 4081 gcctaataa //