##gff-version 3
##sequence-region BBa_K1363400 1 4089
BBa_K1363400	.	engineered_region	209	2004	.	+	0	ID=BBa_K592016;Name=BBa_K592016
BBa_K1363400	.	ribosome_entry_site	1366	1377	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1363400	.	conserved_region	1370	1373	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1363400	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1363400	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1363400	.	CDS	227	1357	.	+	0	ID=BBa_K592004;Name=BBa_K592004;Parent=BBa_K592016
BBa_K1363400	.	CDS	227	1357	.	+	0	ID=annotation2130111;Name=YF1;Parent=BBa_K592004
BBa_K1363400	.	CDS	1384	2004	.	+	0	ID=BBa_K592005;Name=BBa_K592005;Parent=BBa_K592016
BBa_K1363400	.	CDS	1384	2004	.	+	0	ID=annotation2130114;Name=FixJ;Parent=BBa_K592005
BBa_K1363400	.	terminator	2013	2141	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1363400	.	terminator	2013	2092	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1363400	.	engineered_region	2013	2092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1363400	.	stem_loop	2024	2067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1363400	.	terminator	2101	2141	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1363400	.	engineered_region	2101	2141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1363400	.	stop_codon	2134	2134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1363400	.	stem_loop	2108	2127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1363400	.	polyA_site	2128	2141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1363400	.	engineered_region	2150	4089	.	+	0	ID=BBa_K592020;Name=BBa_K592020
BBa_K1363400	.	ribosome_entry_site	3403	3414	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592020
BBa_K1363400	.	conserved_region	3407	3410	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1363400	.	CDS	3421	4089	.	+	0	ID=BBa_K592009;Name=BBa_K592009;Parent=BBa_K592020
BBa_K1363400	.	CDS	3421	4086	.	+	0	ID=annotation2131628;Name=amilCP;Parent=BBa_K592009
BBa_K1363400	.	promoter	2150	2399	.	+	0	ID=BBa_K592006;Name=BBa_K592006;Parent=BBa_K592020
BBa_K1363400	.	promoter	2150	2399	.	+	0	ID=annotation2130179;Name=FixK2 promoter;Parent=BBa_K592006
BBa_K1363400	.	engineered_region	2408	3394	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_K592020
BBa_K1363400	.	promoter	3346	3394	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K1363400	.	promoter	3383	3388	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1363400	.	promoter	3360	3365	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1363400	.	engineered_region	3346	3394	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1363400	.	non_covalent_binding_site	3370	3386	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1363400	.	non_covalent_binding_site	3346	3362	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1363400	.	terminator	3297	3337	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K1363400	.	engineered_region	3297	3337	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1363400	.	stop_codon	3330	3330	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1363400	.	stem_loop	3304	3323	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1363400	.	polyA_site	3324	3337	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1363400	.	CDS	2426	3175	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K1363400	.	tag	3137	3169	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1363400	.	CDS	2429	3136	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1363400	.	engineered_tag	3176	3200	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1363400	.	terminator	3209	3288	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K1363400	.	engineered_region	3209	3288	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1363400	.	stem_loop	3220	3263	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1363400	.	ribosome_entry_site	2408	2419	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K1363400	.	conserved_region	2412	2415	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1363400	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1363400	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1363400	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1363400	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1363400	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1363400	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1363400	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1363400	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_K1363400
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctagttt
tcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattaca
gatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaa
ttttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttaca
aaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgat
ccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtc
tccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacga
gctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaac
acaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcg
acttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggct
tgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtg
cagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcg
tcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcat
tccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatc
gaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccg
acgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgac
gcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcct
ttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagct
gttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtc
gaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcgg
cgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccc
cagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccg
cgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcga
tgcgcgccggcatgctcaacgattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagaggccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccg
cctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggc
actcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcac
ctcaactgctgcgcgcgcactgcgtcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccatta
acacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccagg
aatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctta
taacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgag
atgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggga
tgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattc
tgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtta
attctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttca
agaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagag
ttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgct
ttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctac
tagagaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaa
tggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcacc
aagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtacc
ctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatgg
tgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaac
tttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatg
gaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactactta
caaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggat
tacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa