BBa_K1364003
1
D4E1
RBS - Antifungal D4E1 - Double terminator
2014-09-24T11:00:00Z
2016-02-03T10:04:36Z
De Lucca AJ,??Bland JM,??Grimm C,??Jacks TJ,??Cary JW,??Jaynes JM,??Cleveland TE,??Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
Can J Microbiol.??1998 Jun;44(6):514-20.
D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conidial wall of a variety of fungi.
This part is composed of a RBS (K780002 spoVG), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis.
false
false
_1740_
4206
21465
9
In stock
false
The coding sequence of D4E1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.
false
Jourdan Camille
annotation2386386
1
D4E1
range2386386
1
140
192
annotation2386383
1
BBa_K780002 strong RBS
range2386383
1
1
16
annotation2386384
1
Signal Peptide
range2386384
1
17
97
annotation2386387
1
BBa_B0015
range2386387
1
194
322
annotation2386385
1
Pro Peptide
range2386385
1
98
139
BBa_K1364003_sequence
1
agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagttcaagttacgggcgaaaatcaaagtacggctgcgggcgaagatcaagctatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z