LOCUS       BBa_K1364008             429 bp    DNA     linear       22-Nov-2024
DEFINITION  Pveg - strong RBS - Antifungal GAFP-1 - Double terminator
ACCESSION   BBa_K1364008
VERSION     BBa_K1364008.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..121
                     /label=strong RBS K780002
     CDS             122..202
                     /label=Signal peptide
     CDS             203..244
                     /label=Propeptide
     CDS             245..292
                     /label=GAFP-1
     misc_feature    106..292
                     /label=BBa_K1364002
     stem_loop       312..355
                     /label=stem_loop
     misc_feature    301..380
                     /label=BBa_B0010
     terminator      301..380
                     /label=BBa_B0010
     stem_loop       396..415
                     /label=T7 TE
     misc_feature    422..422
                     /label=stop
     polyA_site      416..429
                     /label=polya
     misc_feature    389..429
                     /label=BBa_B0012
     terminator      389..429
                     /label=BBa_B0012
     terminator      301..429
                     /label=BBa_B0015
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagagaga acaaggaggg
      121 gatgtttgca aagagattta agacgagttt acttccgctg tttgccggct tccttttgct
      181 gtttcacctt gtattagccg gtcctgcggc agcaagtgcg gaaacggcta acaaaagtaa
      241 cgagctggat agcctttcct tttcatataa taatttcgaa gaggacgatt aatactagag
      301 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      361 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      421 gcgtttata
//