LOCUS       BBa_I13501               843 bp    DNA     linear       11-May-2024
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13501
VERSION     BBa_I13501.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 ata
//
LOCUS       BBa_K1364016            1026 bp    DNA     linear       11-May-2024
DEFINITION  PlepA + RFP
ACCESSION   BBa_K1364016
VERSION     BBa_K1364016.1  
FEATURES             Location/Qualifiers
     promoter        1..157
                     /label=BBa_K823002
     RBS             166..177
                     /label=rbs
     RBS             166..177
                     /label=BBa_K143021
     CDS             184..858
                     /label=mrfp1
     misc_feature    865..889
                     /label=Help:Barcodes
     CDS             184..864
                     /label=BBa_E1010
     stem_loop       909..952
                     /label=stem_loop
     misc_feature    898..977
                     /label=BBa_B0010
     terminator      898..977
                     /label=BBa_B0010
     stem_loop       993..1012
                     /label=T7 TE
     misc_feature    1019..1019
                     /label=stop
     polyA_site      1013..1026
                     /label=polya
     misc_feature    986..1026
                     /label=BBa_B0012
     terminator      986..1026
                     /label=BBa_B0012
     terminator      898..1026
                     /label=BBa_B0015
     CDS             184..1026
                     /label=BBa_K606012
     misc_feature    166..1026
                     /label=BBa_K606013
ORIGIN
        1 agtcaatgta tgaatggata cgggatatga atcaataagt acgtgaaaga gaaaagcaac
       61 ccagatatga tagggaactt ttctctttct tgttttacat tgaatcttta caatcctatt
      121 gatataatct aagctagtgt attttgcgtt taatagttac tagagaaagg tggtgaatac
      181 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      241 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      301 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      361 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      421 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      481 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      541 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      601 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      661 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      721 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      781 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      841 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1021 tttata
//