LOCUS BBa_K1364021 5760 bp DNA linear 22-Nov-2024 DEFINITION Integrative plasmid for Bacillus subtilis (pSBbs4E) ACCESSION BBa_K1364021 VERSION BBa_K1364021.1 FEATURES Location/Qualifiers CDS 288..1047 /label=ermB CDS 1181..1736 /label='thrC CDS 3347..4205 /label=amp CDS 4661..5163 /label=thrC' ORIGIN 1 tactagtagc ggccgctgca gatgtggaaa ggacttatcc atcaatataa agaattttta 61 cctgtaacag atcaaacacc ggcgctaact ttacatgaag gaaacacacc tcttattcac 121 ctgccgaagc tgtctgagca gctcggaatt gagcttcatg tcaaaacgga aggcgtcaat 181 cctacgggat catttaaaga tcgcggaatg gttatggctg tggcaaaggc aaaagaagaa 241 ggcaatgaca cgattatgtg cgcgtcaaca ggtaacactt ccgctgctgc ggcagcatat 301 gcagcccgtg ctaacatgaa atgcattgtc atcatcccga acggaaaaat tgcatttgga 361 aaactcgctc aagctgtcat gtacggagcc gagattatcg caattgacgg aaactttgac 421 gatgcgctta aaattgtccg ttccatctgt gagaaatcac cgattgccct tgtcaactca 481 gtcaaccctt accgcattga aggccaaaaa actgctgcct tcgaattcga taaccctaaa 541 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa 601 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag 661 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa 721 cagactaaaa acagtgatta cgcagataaa tacgttagat taattcctac cagtgactaa 781 tcttatgact ttttaaacag ataactaaaa ttacaaacaa atcgtttaac ttctgtattt 841 atttatagat gtatcacttc aggagtgatt acatgaacaa aaatataaaa tattctcaaa 901 actttttaac gaatgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa 961 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa 1021 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa 1081 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc 1141 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta 1201 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat 1261 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct 1321 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca 1381 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat 1441 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc 1501 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc 1561 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt 1621 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt 1681 atactactga cagcttccaa ggagctaaag aggtccctag cgcctacggg gaatttgtat 1741 cgaagtgtgc gaacagctgg gggaagcgcc tgatgtttta gcgatcccgg tcggaaatgc 1801 tggaaacatc actgcgtact ggaagggctt caaggaatat catgagaaaa acggcacagg 1861 ccttccgaaa atgcgcggct ttgaagctga gggtgcggcg gcaatcgtgc gcaatgaagt 1921 gattgaaaat ccggaaacaa tagcgacagc cattcgtatc ggaaacccgg caagctggga 1981 caaagctgta aaggcagccg aggaatccaa tgggaaaatt gacgaagtca ctgatgatga 2041 aatccttcac gcatatcagc tgatcgcccg tgtagaaggc gtgtttgcag aaccaggttc 2101 ttgcgcgtct atcgcaggag tgctgaaaca ggtgaaatcc ggagaaattc cgaaaggcag 2161 caaggtcgta gctgtgttaa caggaaacgg actgaaagat ccgaacacag cggtcgacat 2221 ttcagaaatc aagcctgtca cattgccgac tgatgaagac agcatccttg aatatgtaaa 2281 aggagcggcc cgtgtatgaa cgaagccgac atgctgttct ctgtcactgt tcccggaagc 2341 acagctaacc taggccccgg ctttgattca gtcggaatgg cgctcagcag atatttgaag 2401 ctgaccgtct ttgaaagcga caaatggtct tttgaggctg aaacagaaac agtcgccgga 2461 attaattcga gctcctttaa cttcctcacc agtagttgta tcggtaccat aagtagaagc 2521 agcaacccaa gtagctttac cagcatccgg ttcaaccagc atagtaagaa tcttactgga 2581 catcggcagt tcttcgaaca gtgcgccaac taccagctct ttctgcagtt cattcagggc 2641 accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc 2701 tcttgatcca tggattacgc gttaacccgg gcccgcggat gcatatgatc agatcttaag 2761 gcctaggtct agagtctttg ttttgacgcc attagcgtac gtaacaatcc tcgttaaagg 2821 acaaggacct gagcggaagt gtatcgtaca gtagacggag tatactagta tagtctatag 2881 tccgtggaat tattatattt atctccgacg atattctcat cagtgaaatc cagctggagt 2941 tctttagcaa atttttttat tagctgaact tagtattagt ggccatactc ctccaatcca 3001 aagctattta gaaagattac tatatcctca aacaggcggt aaccggcctc ttcatcggga 3061 atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag tatccagctc 3121 gaggtcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 3181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 3241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 3301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 3361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 3421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 3481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 3541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 3601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tgatagctct tgatccggca 3661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 3721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 3781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 3901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 4021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 4081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 4141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 4201 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 4261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 4321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 4381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 4441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 4501 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 4561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 4621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 4681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 4741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 4801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 4921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc 4981 atgtaaaaga tgaggttggt tcattctcga aaattacatc tgtgttctca gagcggggcg 5041 tgacgtttga aaaaatcctt cagctgccaa ttaaaggcca tgatgagtta gctgaaatcg 5101 taattgtcac acatcataca tcagaagctg atttcagtga tatcctgcaa aacctaaatg 5161 atttggaagt cgttcaagaa gtcaaaagca catatcgtgt agaagggaac ggttggagct 5221 aatgtggaaa ggacttatcc atcaatataa agaattttta cctgtaacag atcaaacacc 5281 ggcgctaact ttacatgaag gaaacacacc tcttattcac ctgccgaagc tgtctgagca 5341 gctcggaatt gagcttcatg tcaaaacgga aggcgtcaat cctacgggat catttaaaga 5401 tcgcggaatg gttatggctg tggcaaaggc aaaagaagaa ggcaatgaca cgattatgtg 5461 cgcgtcaaca ggtaacactt ccgctgctgc ggcagcatat gcagcccgtg ctaacatgaa 5521 atgcattgtc atcatcccga acggaaaaat tgcatttgga aaactcgctc aagctgtcat 5581 gtacggagcc gagattatcg caattgacgg aaactttgac gatgcgctta aaattgtccg 5641 ttccatctgt gagaaatcac cgattgccct tgtcaactca gtcaaccctt accgcattga 5701 aggccaaaaa actgctgcct tcggatccat taccgccgga attcgcggcc gcttctagag //