BBa_K1365003
1
BBa_K1365003
NisC
2014-09-23T11:00:00Z
2015-05-08T01:10:07Z
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.
NisC catalyzes the ring formation in the nisin precursor protein. Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis.
false
false
_1741_
0
23000
9
It's complicated
false
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC-3'
to PCR NisC from the genome and add the BioBrick prefix and suffix.
false
Sandra Mous
annotation2386155
1
start
range2386155
1
1
3
annotation2386157
1
NisC
range2386157
1
1
1245
annotation2386156
1
stop
range2386156
1
1243
1245
BBa_K1365003_sequence
1
atgaataaaaaaaatataaaaagaaatgttgaaaaaattattgctcaatgggatgagagaactagaaaaaataaagaaaacttcgatttcggagagttgactctctctacaggattgcctggtataattttaatgttagcggagttaaaaaataaagataactcaaagatatatcagaaaaagatagacaattatattgaatatattgttagcaaactttcaacatatgggcttttaacaggatcactttattcgggagcagctggcattgcattaagtatcctacatttacgagaagatgacgaaaaatataagaatcttcttgatagcctaaatagatatatcgaatatttcgtcagagaaaaaattgaaggatttaatttggaaaacattactcctcctgattatgacgtgattgaaggtttatctgggatactttcctatctattattaatcaacgacgagcaatatgatgatttgaaaatactcattatcaattttttatcaaatctgactaaagaaaacaatggactaatatcgctttacatcaaatcggagaaacagatgtctcaatcagaaagtgagatgtatccactaggctgtttgaatatgggattagcacatggacttgctggagtgggctgtatcttagcttatgcccacataaaaggatatagtaatgaagcctcgttgtcagctttgcaaaaaattatttttatttatgaaaagtttgaacttgaaaggaaaaaacagtttctatggaaagatggacttgtagcagatgaattaaaaaaagagaaagtaattagggaagcaagtttcattagagatgcatggtgctatggaggtccaggtattagtctgctatacttatacggaggattagcactggataatgactattttgtagataaagcagaaaaaatattagagtcagctatgcaaaggaaacttggtattgattcatatatgatttgccatggctattctggtttaatagaaatttgttctttatttaagcggctattaaatacaaaaaagtttgattcatacatggaagaatttaatgttaatagtgagcaaattcttgaagaatacggagatgaaagtggcacgggttttcttgaaggaataagtggctgtatactggtattatcgaaatttgaatattcaatcaattttacttattggagacaagcactgttactttttgacgattttttgaaaggagggaagaggaaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z