BBa_K1365003 1 BBa_K1365003 NisC 2014-09-23T11:00:00Z 2015-05-08T01:10:07Z Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain. NisC catalyzes the ring formation in the nisin precursor protein. Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis. false false _1741_ 0 23000 9 It's complicated false Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC-3' to PCR NisC from the genome and add the BioBrick prefix and suffix. false Sandra Mous annotation2386155 1 start range2386155 1 1 3 annotation2386157 1 NisC range2386157 1 1 1245 annotation2386156 1 stop range2386156 1 1243 1245 BBa_K1365003_sequence 1 atgaataaaaaaaatataaaaagaaatgttgaaaaaattattgctcaatgggatgagagaactagaaaaaataaagaaaacttcgatttcggagagttgactctctctacaggattgcctggtataattttaatgttagcggagttaaaaaataaagataactcaaagatatatcagaaaaagatagacaattatattgaatatattgttagcaaactttcaacatatgggcttttaacaggatcactttattcgggagcagctggcattgcattaagtatcctacatttacgagaagatgacgaaaaatataagaatcttcttgatagcctaaatagatatatcgaatatttcgtcagagaaaaaattgaaggatttaatttggaaaacattactcctcctgattatgacgtgattgaaggtttatctgggatactttcctatctattattaatcaacgacgagcaatatgatgatttgaaaatactcattatcaattttttatcaaatctgactaaagaaaacaatggactaatatcgctttacatcaaatcggagaaacagatgtctcaatcagaaagtgagatgtatccactaggctgtttgaatatgggattagcacatggacttgctggagtgggctgtatcttagcttatgcccacataaaaggatatagtaatgaagcctcgttgtcagctttgcaaaaaattatttttatttatgaaaagtttgaacttgaaaggaaaaaacagtttctatggaaagatggacttgtagcagatgaattaaaaaaagagaaagtaattagggaagcaagtttcattagagatgcatggtgctatggaggtccaggtattagtctgctatacttatacggaggattagcactggataatgactattttgtagataaagcagaaaaaatattagagtcagctatgcaaaggaaacttggtattgattcatatatgatttgccatggctattctggtttaatagaaatttgttctttatttaagcggctattaaatacaaaaaagtttgattcatacatggaagaatttaatgttaatagtgagcaaattcttgaagaatacggagatgaaagtggcacgggttttcttgaaggaataagtggctgtatactggtattatcgaaatttgaatattcaatcaattttacttattggagacaagcactgttactttttgacgattttttgaaaggagggaagaggaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z