BBa_K1365004 1 BBa_K1365004 NisI 2014-09-23T11:00:00Z 2015-05-08T01:10:07Z Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain. NisI is a nisin immunity gene. In Lactococcus lactis it protects against it's own nisin. false false _1741_ 0 23000 9 No part sequence false Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG-3' to PCR NisI from the genome and add the BioBrick prefix and suffix. Deleted illegal SpeI site at position 762 using above primers, changing a AAC codon in a AAT. false Sandra Mous igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z