BBa_K1365004
1
BBa_K1365004
NisI
2014-09-23T11:00:00Z
2015-05-08T01:10:07Z
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.
NisI is a nisin immunity gene. In Lactococcus lactis it protects against it's own nisin.
false
false
_1741_
0
23000
9
No part sequence
false
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG-3'
to PCR NisI from the genome and add the BioBrick prefix and suffix.
Deleted illegal SpeI site at position 762 using above primers, changing a AAC codon in a AAT.
false
Sandra Mous
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z