BBa_K1365007 1 BBa_K1365007 NisF, NisE and NisG 2014-09-23T11:00:00Z 2015-05-08T01:10:07Z Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain. Together with NisI, the genes NisF, NisE and NisG provide immunity against nisin for Lactococcus lactis. false false _1741_ 0 23000 9 No part sequence false Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT-3' to PCR NisF, NisE and NisG from the genome and add the BioBrick prefix and suffix. The illegal PstI site at position 186, illegal EcoRI site at postion 336 and illegal XbaI site at position 349 were removed using primers as well, fusing the resulting PCR fragments together again with a Gibson assembly. false Sandra Mous igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z