LOCUS       BBa_K1365120            2350 bp    DNA     linear       06-Dec-2025
DEFINITION  anti Pseudomonas aeruginosa secreting system
ACCESSION   BBa_K1365120
VERSION     BBa_K1365120.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     misc_feature    184..264
                     /label=ssUSP45
     CDS             265..1347
                     /label=dspB
     misc_feature    1348..1353
                     /label=stop
     CDS             184..1353
                     /label=BBa_K1365111
     misc_feature    1366..1369
                     /label=conserved
     RBS             1362..1373
                     /label=BBa_B0034
     misc_feature    1380..1459
                     /label=ssUSP45
     CDS             1460..2206
                     /label=aiiA
     misc_feature    2207..2212
                     /label=TAATAA
     misc_feature    1380..1380
                     /label=start
     CDS             1380..2213
                     /label=BBa_K1365169
     stem_loop       2233..2276
                     /label=stem_loop
     misc_feature    2222..2301
                     /label=BBa_B0010
     terminator      2222..2301
                     /label=BBa_B0010
     stem_loop       2317..2336
                     /label=T7 TE
     misc_feature    2343..2343
                     /label=stop
     polyA_site      2337..2350
                     /label=polya
     misc_feature    2310..2350
                     /label=BBa_B0012
     terminator      2310..2350
                     /label=BBa_B0012
     terminator      2222..2350
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgaaaa aaaagattat ctcagctatt ttaatgtcta cagtgatact ttctgctgct
      241 gccccgttgt caggtgttta cgctaattgt tgcgtaaaag gcaattccat atatccgcaa
      301 aaaacaagta ccaagcagac cggattaatg ctggacatcg cccgacattt ttattcaccc
      361 gaggtgatta aatcctttat tgataccatc agcctttccg gcggtaattt tctgcacctg
      421 catttttccg accatgaaaa ctatgcgata gaaagccatt tacttaatca acgtgcggaa
      481 aatgccgtgc agggcaaaga cggtatttat attaatcctt ataccggaaa gccattcttg
      541 agttatcggc aacttgacga tatcaaagcc tatgctaagg caaaaggcat tgagttgatt
      601 cccgaacttg acagcccgaa tcacatgacg gcgatcttta aactggtgca aaaagacaga
      661 ggggtcaagt accttcaagg attaaaatca cgccaggtag atgatgaaat tgatattact
      721 aatgctgaca gtattacttt tatgcaatct ttaatgagtg aggttattga tatttttggc
      781 gacacgagtc agcattttca tattggtggc gatgaatttg gttattctgt ggaaagtaat
      841 catgagttta ttacgtatgc caataaacta tcctactttt tagagaaaaa agggttgaaa
      901 acccgaatgt ggaatgacgg attaattaaa aatacttttg agcaaatcaa cccgaatatt
      961 gaaattactt attggagcta tgatggcgat acgcaggaca aaaatgaagc tgccgagcgc
     1021 cgtgatatgc gggtcagttt gccggagttg ctggcgaaag gctttactgt cctgaactat
     1081 aattcctatt atctttacat tgttccgaaa gcttcaccaa ccttctcgca agatgccgcc
     1141 tttgccgcca aagatgttat aaaaaattgg gatcttggtg tttgggatgg acgaaacacc
     1201 aaaaaccgcg tacaaaatac tcatgaaata gccggcgcag cattatcgat ctggggagaa
     1261 gatgcaaaag cgctgaaaga cgaaacaatt cagaaaaaca cgaaaagttt attggaagcg
     1321 gtgattcata agacgaatgg ggatgactaa taatactaga gaaagaggag aaatactaga
     1381 tgaaaaaaaa gattatctca gctattttaa tgtctacagt gatactttct gctgctgccc
     1441 cgttgtcagg tgtttacgct acagtaaaga agctttattt cgtcccagca ggtcgttgta
     1501 tgttggatca ttcgtctgtt aatagtacat taacaccagg agaattatta gacttaccgg
     1561 tttggtgtta tcttttggag actgaagaag gacctatttt agtagataca ggtatgccag
     1621 aaagtgcagt taataatgaa ggtcttttta acggtacatt tgtcgaaggg caggttttac
     1681 cgaaaatgac tgaagaagat agaatcgtga atattttaaa acgggttggt tatgagccgg
     1741 aagaccttct ttatattatt agttctcact tgcattttga tcatgcagga ggaaatggcg
     1801 cttttataaa tacaccaatc attgtacagc gtgctgaata tgaggcggcg cagcatagcg
     1861 aagaatattt gaaagaatgt atattgccga atttaaacta caaaatcatt gaaggtgatt
     1921 atgaagtcgt accaggagtt caattattgc atacaccagg ccatactcca gggcatcaat
     1981 cgctattaat tgagacagaa aaatccggtc ctgtattatt aacgattgat gcatcgtata
     2041 cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa ttagctttat
     2101 cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt ttctttggac
     2161 atgatataga gcaggaaagg ggatgtaaag tgttccctga atatatataa taatactaga
     2221 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2281 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2341 tgcgtttata
//