LOCUS       BBa_K1365130            2416 bp    DNA     linear       22-Nov-2024
DEFINITION  anti Pseudomonas aeruginosa analytical secreting system
ACCESSION   BBa_K1365130
VERSION     BBa_K1365130.1  
FEATURES             Location/Qualifiers
     promoter        1..159
                     /label=R0079
     RBS             166..177
                     /label=B0034
     CDS             184..1371
                     /label=ssUSP45DspB + tag
     misc_feature    1348..1365
                     /label=6X histidine tag
     misc_feature    1366..1371
                     /label=stop
     RBS             1380..1391
                     /label=B0034
     CDS             1398..2279
                     /label=ssUSP45aiiA
     misc_feature    2256..2273
                     /label=6X histidine tag
     misc_feature    2274..2279
                     /label=stop
     stem_loop       2288..2416
                     /label=B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgaaaa aaaagattat ctcagctatt ttaatgtcta cagtgatact ttctgctgct
      241 gccccgttgt caggtgttta cgctaattgt tgcgtaaaag gcaattccat atatccgcaa
      301 aaaacaagta ccaagcagac cggattaatg ctggacatcg cccgacattt ttattcaccc
      361 gaggtgatta aatcctttat tgataccatc agcctttccg gcggtaattt tctgcacctg
      421 catttttccg accatgaaaa ctatgcgata gaaagccatt tacttaatca acgtgcggaa
      481 aatgccgtgc agggcaaaga cggtatttat attaatcctt ataccggaaa gccattcttg
      541 agttatcggc aacttgacga tatcaaagcc tatgctaagg caaaaggcat tgagttgatt
      601 cccgaacttg acagcccgaa tcacatgacg gcgatcttta aactggtgca aaaagacaga
      661 ggggtcaagt accttcaagg attaaaatca cgccaggtag atgatgaaat tgatattact
      721 aatgctgaca gtattacttt tatgcaatct ttaatgagtg aggttattga tatttttggc
      781 gacacgagtc agcattttca tattggtggc gatgaatttg gttattctgt ggaaagtaat
      841 catgagttta ttacgtatgc caataaacta tcctactttt tagagaaaaa agggttgaaa
      901 acccgaatgt ggaatgacgg attaattaaa aatacttttg agcaaatcaa cccgaatatt
      961 gaaattactt attggagcta tgatggcgat acgcaggaca aaaatgaagc tgccgagcgc
     1021 cgtgatatgc gggtcagttt gccggagttg ctggcgaaag gctttactgt cctgaactat
     1081 aattcctatt atctttacat tgttccgaaa gcttcaccaa ccttctcgca agatgccgcc
     1141 tttgccgcca aagatgttat aaaaaattgg gatcttggtg tttgggatgg acgaaacacc
     1201 aaaaaccgcg tacaaaatac tcatgaaata gccggcgcag cattatcgat ctggggagaa
     1261 gatgcaaaag cgctgaaaga cgaaacaatt cagaaaaaca cgaaaagttt attggaagcg
     1321 gtgattcata agacgaatgg ggatgagcac catcaccatc accattaata atactagaga
     1381 aagaggagaa atactagatg aaaaaaaaga ttatctcagc tattttaatg tctacagtga
     1441 tactttctgc tgctgccccg ttgtcaggtg tttacgctgc tgctgccccg ttgtcaggtg
     1501 tttacgctac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg ttggatcatt
     1561 cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt tggtgttatc
     1621 ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa agtgcagtta
     1681 ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg aaaatgactg
     1741 aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa gaccttcttt
     1801 atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct tttataaata
     1861 caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa gaatatttga
     1921 aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat gaagtcgtac
     1981 caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg ctattaattg
     2041 agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg aaagagaatt
     2101 ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct tcaattaaac
     2161 gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat gatatagagc
     2221 aggaaagggg atgtaaagtg ttccctgaat atatacacca tcaccatcac cattaataat
     2281 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2341 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2401 cctttctgcg tttata
//