LOCUS BBa_K1365301 6230 bp DNA linear 25-Nov-2024 DEFINITION pIL253-mRFP Biobrick Vector for L.lactis ACCESSION BBa_K1365301 VERSION BBa_K1365301.1 FEATURES Location/Qualifiers primer_bind 1369..1388 /label=G00101 (VR) primer_bind 6..25 /label=G00100 (VF2) misc_feature 146..1214 /label=J04450 (mRFP Construct) misc_feature 1885..3375 /label=Origin of Replication misc_feature 4368..4838 /label=ORF3 CDS 4959..5696 /label=Erythromycin resistance misc_feature 146..345 /label=R0010 (LacI reg. promoter) CDS 146..233 /label=end of LacI coding region (inactive) misc_feature 234..271 /label=CAP binding site promoter 282..287 /label=-35 promoter 306..311 /label=-10 misc_feature 311..345 /label=LacI binding site misc_feature 318..318 /label=start RBS 354..365 /label=B0034 (RBS) misc_feature 372..372 /label=start CDS 372..1046 /label=E1010 (mRFP) misc_feature 1050..1050 /label=stop misc_feature 1047..1047 /label=stop misc_feature 1086..1214 /label=B0015 stem_loop 1087..1143 /label=B0010 stem_loop 1181..1200 /label=B0012 misc_feature 124..145 /label=prefix misc_feature 1215..1235 /label=suffix misc_feature 5696..5696 /label=start ORIGIN 1 ctaggtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt 121 ctggaattcg cggccgcttc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg 181 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 241 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 301 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg 361 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc 421 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc 481 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg 541 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg 601 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta 661 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg 721 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc 781 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc 841 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag 901 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca 961 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg 1021 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac 1081 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1141 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1201 tttctgcgtt tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca 1261 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg 1321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 1381 gcggtaatca attctattta atcactttga ctagcaaata ctaacaacaa gacacacaca 1441 ccaaaaatca aaaattcact acttttagtt aaaaaccacg taaccacaag aactaatcca 1501 atccatgtaa tcgggttctt caaatatttc tccaagattt tcctcctcta atatgctcaa 1561 cttaaatgac ctattcaata aatctattat gctgctaaat agtttatagg acaaataagt 1621 atactctaat gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc ttcgctctca 1681 ctgcccctcg acgttttagt agcctttccc tcacttcgtt cagtccaagc caactaaaag 1741 ttttcgggct actctctcct tctcccccta ataattaatt aaaatcttac tctgtatatt 1801 tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag atataaatgt 1861 aatggcatag tgcgggtttt attttcagcc tgtatcatag ctaaacaaat cgagttgtgt 1921 gtccgtttta gggcgttctg ctagcttgtt taaagtctct tgaatgaatg tatgctctaa 1981 gtcaaaagaa tttgtcagcg cctttatata gctttctttt tcttcttttt ttactttaat 2041 gatcgatagc aacaatgatt taacactagc aagttgaatg ccaccatttc ttcctggttt 2101 aatcttaaag aaaatttcct gattcgcctt cagtaccttc agcaatttat ctaatgtccg 2161 ttcaggaatg cctagcactt ctctaatctc ttttttggtc gtcactaaat aaggcttgta 2221 tacatcgctt ttttcgctaa tataagccat taaatcttct ttccattctg acaaatgaac 2281 acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga caaataaatc 2341 tttactggtt aaatcacttg atacccaagc tttgcaaaga atggtaatgt attccctatt 2401 agccccttga tagttttctg aataggcact tctaacaatt ttgattactt ctttttcttc 2461 taagggttga tctaatcgat tattaaactc aaacatatta tattcgcacg tttcgattga 2521 atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg acgttattac gccctattaa 2581 acccttttct cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg gttcatctac 2641 ttgttttttg ccttctgtac cgcttaaaac cgttagactt gaacgagtaa agcccttatt 2701 atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa gaataacggt aattaggatc 2761 aaaaaattct acattgtccg ttcttggtat gcgagcaata ccaaaatgat tacacgttag 2821 atcaactggc aaagactttc caaaatattc tcggatattt tgcgaaatta ttttggctgc 2881 tttgacagat ttaaattctg attttgaagt cacatagact ggcgtttcta aaacaaaata 2941 tgcttgataa cctttatcag atttgataat catagtaggc ataaaaccta aatcaatagc 3001 ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat caaaatcaat 3061 aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt atgaacggtt 3121 ttcgtctgca tacgttccat aacgataaac gttgggtgtc caatgtgtaa atgtatcttg 3181 attttcttga atcgcttcct cggaagtcag aacaacacca cgaccgccaa tcatgcttga 3241 ttttgagcga tacgcaaaaa tagccccttt gcttttacct ggcttggtag tgattgagcg 3301 aattttacta tttttaaatt tgtactttaa caagccgtca tgaagcacag tttctacaac 3361 aaaagggata ttcattcagc tgttctcctt tcctataaat cctataaaat aggttgttta 3421 attaacttgg tttgcttttt cattcaactg tttcaatatt gcatgttttg aaaaagattt 3481 ttttccttta taagtcaatt tttttccact aatcgaataa attattttgt tattttctat 3541 taacttatat atataatctt ccccctccga agaaaaatac ttatctgatt ttgtttctaa 3601 gtagatattt ctcttttcta actctttctt aaacgtttct agtgtataga tatttgctaa 3661 ttttcttatc tccaataaac tattttttat ataagtttta cattcatcat gattcataca 3721 aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg attgattgct 3781 taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc cctatataat 3841 gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct tacgagggaa 3901 gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct atgcatttat 3961 aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc tattcagttc 4021 gttgtttcgt tatatctagt ataccgcttt taaaaaaaaa taagcaacga tttcgtgcat 4081 tattcacacg aagtcattgc ttttttcttc ttccatttct aaatccaatg ttacttgttc 4141 tgattctgtt tctggctctg gttctgttgg ctcatttggg attaaatcca ctactagcgt 4201 tgagttagtt aactttgcaa tttgttctag tgtttttatg gttggatctg attttcctga 4261 ttctattcgt gaataatttg atctactcat ttctaattct tggggtaccg ccagcatttc 4321 ggaaaaaaac cacgctaagg attttttcta taaaaagagc cgttatatta agaataaaac 4381 ggctctttta tacgtaaagg acgtaaattc atttgcccag tgtcatgtaa tccttcaaat 4441 ttgtattctc caagaaaatt gatatgttcc catcctaacg gccacgcata tggcattaaa 4501 tcttctctaa attctcctct tgcttttaat tcttctacgg ctttttccat atatacagtg 4561 ttccacacac ttatagcgtt aataattatg tttagtgcac tagctctttg taactggtct 4621 tggagagcac gttctctaaa ttctccacgt tgtccaaaaa atatagttct agctaatgca 4681 ttgattgctt ctcctttatt taaacctttt tgaacccgtc tccttacggc tttattagat 4741 atgtaatcca gcgtaaagag ggttttctcg attcgtccca tttctccaag tgctgttgcg 4801 agtttatttt gtcttgcata tgatccgagc ttccccatga taagagcgct agggacctct 4861 ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc cctttagtaa 4921 cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc gttaaataat 4981 agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa attgtttact 5041 ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg atattctcga 5101 ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg aacatctgtg 5161 gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg aaagcattcc 5221 gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt 5281 cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata gatgtcagac 5341 gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta aggaatactc 5401 ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc ttggtgaatt 5461 aaagtgacac gagtattcag ttttaatttt tctgacgata agttgaatag atgactgtct 5521 aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa atgcccttta 5581 cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt tattatttgg 5641 ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt gttcatgtaa 5701 tcactccttc ttaattacaa atttttagca tctaatttaa cttcaattcc tattatacaa 5761 aattttaaga tactgcacta tcaacacact cttaagtttg cttctaagtc ttatttccat 5821 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc 5881 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc 5941 atttcgtttt tcttttgcgc ttcttgataa aagggatagt aattcattcc tggttgcaaa 6001 ttttgaaaac cgctacggat cacatctttt tctaaactat tgatccatag tcttttatac 6061 gttttatctt tagaaaaggc atttgcttta tgaatgatcg accaggcaat gttttcgcct 6121 tctctgtcgc tatctgttgc gacaatgatt gtatttgctt gttttaaaag ttcagcaaca 6181 attttaaact gctttttttt atctgttgcc acttcaaaat cgtatcgatt //