LOCUS       BBa_K1366021            1269 bp    DNA     linear       22-Nov-2024
DEFINITION  Genetic construct for lpp gen deletion
ACCESSION   BBa_K1366021
VERSION     BBa_K1366021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..50
                     /label=Lpp 50bp homology (1)
     misc_feature    51..84
                     /label=FRT
     promoter        85..113
                     /label=AmpR promoter
     RBS             114..126
                     /label=RBS
     CDS             127..982
                     /label=AmpR
     misc_feature    983..1112
                     /label=Double terminator
     misc_feature    1113..1146
                     /label=FRT
     misc_feature    1217..1267
                     /label=Lpp 50pb homology (2)
     misc_feature    1147..1216
                     /label=MCS
ORIGIN
        1 agctttgtgt aatacttgta acgctacatg gagattaact caatctagag gggaagttcc
       61 tatacttttt agagaatagg aacttcttca aatatgtatc cgctcatgag acaataaccc
      121 tgataaatgc ttcaataata ttgaaaaagg aagagtaaag aggagaaaat gagtattcaa
      181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      241 ccagaaacgc tggtgaaagt aaaagatgcc gaagatcagt tgggtgcacg tgtgggttac
      301 atcgaactgg acctcaacag cggtaagatt cttgagagtt ttcgccccga agaacgtttc
      361 ccaatgatga gcacttttaa agttctgctc tgtggcgcgg tattatcccg tattgacgcc
      421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      481 ccagtcacag aaaagcatct tacggacggc atgacagtac gcgaattatg cagcgctgcc
      541 ataaccatga gtgataacac ggcggccaac ttacttctga caacgatcgg aggaccgaag
      601 gagcttaccg cttttttgca caacatgggt gatcatgtaa ctcgccttga tcgttgggaa
      661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagctatg
      721 gcaacaacgt tgcgcaaact cttaactggc gaacttctta ctctcgcttc ccggcaacaa
      781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtattatt
      901 gcagccctgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagc
      961 caggcaacta tggacgaacg taatcgccag atcgctgaga taggtgcctc cctgattaag
     1021 cattggtaat aaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1081 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1141 gtgggccttt ctgcgtttat agaagttcct atacttttta gagaatagga acttcaagct
     1201 tggatccgtc gacggtaccc acattgtgcg ccattttttt tgtctgccgt ttaccgctac
     1261 tgcgtcacg
//