BBa_K1366021 1 BBa_K1366021 Genetic construct for lpp gen deletion 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015 This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase. false false _1743_ 0 16722 9 It's complicated false This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp false Eduardo Cepeda Ca??edo annotation2414665 1 Double terminator range2414665 1 983 1112 annotation2414666 1 FRT range2414666 1 1113 1146 annotation2414682 1 MCS range2414682 1 1147 1216 annotation2414661 1 FRT range2414661 1 51 84 annotation2414664 1 AmpR range2414664 1 127 982 annotation2414660 1 Lpp 50bp homology (1) range2414660 1 1 50 annotation2414681 1 Lpp 50pb homology (2) range2414681 1 1217 1267 annotation2414663 1 RBS range2414663 1 114 126 annotation2414662 1 AmpR promoter range2414662 1 85 113 BBa_K1366021_sequence 1 agctttgtgtaatacttgtaacgctacatggagattaactcaatctagaggggaagttcctatactttttagagaataggaacttcttcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtaaagaggagaaaatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagaagttcctatactttttagagaataggaacttcaagcttggatccgtcgacggtacccacattgtgcgccattttttttgtctgccgtttaccgctactgcgtcacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z