BBa_K1366021
1
BBa_K1366021
Genetic construct for lpp gen deletion
2014-10-09T11:00:00Z
2015-05-08T01:10:07Z
The genetic design is based in the lpp deletion done by:
Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356.
It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015
This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.
false
false
_1743_
0
16722
9
It's complicated
false
This is the general structure
50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp
false
Eduardo Cepeda Ca??edo
annotation2414662
1
AmpR promoter
range2414662
1
85
113
annotation2414660
1
Lpp 50bp homology (1)
range2414660
1
1
50
annotation2414663
1
RBS
range2414663
1
114
126
annotation2414661
1
FRT
range2414661
1
51
84
annotation2414666
1
FRT
range2414666
1
1113
1146
annotation2414682
1
MCS
range2414682
1
1147
1216
annotation2414665
1
Double terminator
range2414665
1
983
1112
annotation2414681
1
Lpp 50pb homology (2)
range2414681
1
1217
1267
annotation2414664
1
AmpR
range2414664
1
127
982
BBa_K1366021_sequence
1
agctttgtgtaatacttgtaacgctacatggagattaactcaatctagaggggaagttcctatactttttagagaataggaacttcttcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtaaagaggagaaaatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagaagttcctatactttttagagaataggaacttcaagcttggatccgtcgacggtacccacattgtgcgccattttttttgtctgccgtttaccgctactgcgtcacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z