LOCUS       BBa_K1366022            1173 bp    DNA     linear       23-Dec-2024
DEFINITION  Genetic construct for msbB gen deletion
ACCESSION   BBa_K1366022
VERSION     BBa_K1366022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..50
                     /label=msbB homology arm (1)
     misc_feature    51..85
                     /label=FRT
     promoter        86..131
                     /label=KanR promoter
     RBS             132..144
                     /label=RBS
     CDS             145..940
                     /label=KanR
     misc_feature    940..1069
                     /label=Double terminator
     misc_feature    1123..1173
                     /label=msbB homology arm (2)
     misc_feature    1070..1104
                     /label=FRT
     misc_feature    1105..1122
                     /label=MSC
ORIGIN
        1 caagttgcgc cgctacacta tcaccagatt gatttttgcc ttatccgaaa gaagttccta
       61 tactttttag agaataggaa cttccattat tgcaattaat aaacaactaa cggacaattc
      121 tacctaacaa aagaggagaa aatgattgaa caagatggat tgcacgcagg ttctccggcc
      181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      301 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
      361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      421 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      661 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
      721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      841 ggcgaatggg ctgaccgctt cctcgtgctc tacggtatcg ccgctcccga ttcgcagcgc
      901 atcgccttct atcgccttct tgacgagttc ttctgaccag gcatcaaata aaacgaaagg
      961 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1021 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatagaagt tcctatactt
     1081 tttagagaat aggaacttca agcttggatc cgtcgacggt acccgcgagg agaggccttc
     1141 gcctgatgat aagttcaagt ttgcttcaga ata
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