BBa_K1366022 1 BBa_K1366022 Genetic construct for msbB gen deletion 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z We obtain the sequence from NCBI. The accession number is NC_007635.1 This biobrick contains the sequence homologies (50bp) to delete msbB gene (LPS moiety carrier) in E. coli with a kanamycin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gene in E. coli). The deletion of msbB gene and the replacement of that sequence with the Kan resistance are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance after the recombination with a specific recombinase. false false _1743_ 0 16722 9 Not in stock false This is the general structure 50bp homology msbB// FRT // KanR Promoter // RBS // KanR // Terminator // FRT // MCS // 50 bp homology msbB false Eduardo Cepeda Ca??edo annotation2414801 1 msbB homology arm (2) range2414801 1 1123 1173 annotation2414796 1 FRT range2414796 1 51 85 annotation2414795 1 msbB homology arm (1) range2414795 1 1 50 annotation2414834 1 FRT range2414834 1 1070 1104 annotation2414876 1 MSC range2414876 1 1105 1122 annotation2414797 1 KanR promoter range2414797 1 86 131 annotation2414800 1 Double terminator range2414800 1 940 1069 annotation2414798 1 RBS range2414798 1 132 144 annotation2414799 1 KanR range2414799 1 145 940 BBa_K1366022_sequence 1 caagttgcgccgctacactatcaccagattgatttttgccttatccgaaagaagttcctatactttttagagaataggaacttccattattgcaattaataaacaactaacggacaattctacctaacaaaagaggagaaaatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctctacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagaagttcctatactttttagagaataggaacttcaagcttggatccgtcgacggtacccgcgaggagaggccttcgcctgatgataagttcaagtttgcttcagaata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z