BBa_K1366101 1 BBa_K1366101 Genetic construct for lpp gene deletion (C1) 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015 This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase. false false _1743_ 0 16722 9 It's complicated false This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp false Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu annotation2427290 1 MCS range2427290 1 1164 1199 annotation2427284 1 50 bp lpp homology (1) range2427284 1 1 50 annotation2427288 1 AmpR range2427288 1 170 1034 annotation2427292 1 50 bp lpp homology (2) range2427292 1 1235 1285 annotation2427289 1 BBa_B0015 range2427289 1 1035 1163 annotation2427287 1 BBa_B0034 range2427287 1 157 169 annotation2427291 1 FRT range2427291 1 1200 1234 annotation2427285 1 FRT range2427285 1 51 84 annotation2427286 1 AmpR promoter range2427286 1 86 156 BBa_K1366101_sequence 1 agctttgtgtaatacttgtaacgctacatggagattaactcaatctagaggggaagttcctatactttttagagaataggaacttcttcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtaaagaggagaaaatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagaagttcctatactttttagagaataggaacttcaagcttggatccgtcgacggtacccacattgtgcgccattttttttgtctgccgtttaccgctactgcgtcacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z