LOCUS       BBa_K1366102            1175 bp    DNA     linear       22-Dec-2024
DEFINITION  Genetic construct for msbB gene deletion (C2)
ACCESSION   BBa_K1366102
VERSION     BBa_K1366102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..50
                     /label=msbB homology arm
     misc_feature    51..85
                     /label=FRT
     promoter        86..131
                     /label=Kan promoter
     RBS             132..144
                     /label=RBS
     CDS             145..940
                     /label=KanR
     misc_feature    941..1070
                     /label=Double terminator
     misc_feature    1071..1105
                     /label=FRT
     misc_feature    1260..1310
                     /label=msbB homology arm
     misc_feature    1106..1259
                     /label=MCS
ORIGIN
        1 agcaagttgc gccgctacac tatcaccaga ttgatttttg ccttatccga aagaagttcc
       61 tatacttttt agagaatagg aacttccatt attgcaatta ataaacaact aacggacaat
      121 tctacctaac aaaagaggag aaaatgattg aacaagatgg attgcacgca ggttctccgg
      181 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      241 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      301 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
      361 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      421 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      481 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      541 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      601 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      661 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
      721 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      781 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      841 gcggcgaatg ggctgaccgc ttcctcgtgc tctacggtat cgccgctccc gattcgcagc
      901 gcatcgcctt ctatcgcctt cttgacgagt tcttctgacc aggcatcaaa taaaacgaaa
      961 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1021 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatagaa gttcctatac
     1081 tttttagaga ataggaactt caagcttgga tccgtcgacg gtacccgcga ggagaggcct
     1141 tcgcctgatg ataagttcaa gtttgcttca gaata
//