BBa_K1366102 1 BBa_K1366102 Genetic construct for msbB gene deletion (C2) 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015 This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase. false false _1743_ 0 16722 9 It's complicated false This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp false Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu annotation2417911 1 FRT range2417911 1 51 85 annotation2417913 1 RBS range2417913 1 132 144 annotation2417917 1 msbB homology arm range2417917 1 1260 1310 annotation2417918 1 MCS range2417918 1 1106 1259 annotation2417916 1 FRT range2417916 1 1071 1105 annotation2417912 1 Kan promoter range2417912 1 86 131 annotation2417910 1 msbB homology arm range2417910 1 1 50 annotation2417915 1 Double terminator range2417915 1 941 1070 annotation2417914 1 KanR range2417914 1 145 940 BBa_K1366102_sequence 1 agcaagttgcgccgctacactatcaccagattgatttttgccttatccgaaagaagttcctatactttttagagaataggaacttccattattgcaattaataaacaactaacggacaattctacctaacaaaagaggagaaaatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctctacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagaagttcctatactttttagagaataggaacttcaagcttggatccgtcgacggtacccgcgaggagaggccttcgcctgatgataagttcaagtttgcttcagaata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z