BBa_K1366104 1 C5 Apoptin expression under a CMV promoter (C5) 2014-10-09T11:00:00Z 2015-05-08T01:10:07Z Yes, we use NCBI to retrieve the DNA sequence. The accession number is NC_001427.1 This construct will be used to characterize the transfection of a death effector (apoptin) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. Apoptin will be codon optimized for mammalian cell line. false false _1743_ 0 16721 9 It's complicated false The sequence design goes like this CMV Promoter+RBS // Apoptin // terminator false Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu annotation2415736 1 hGH terminator range2415736 1 1041 1522 annotation2415737 1 BBa_K404108 range2415737 1 1041 1522 annotation2415738 1 Apoptin range2415738 1 655 1040 annotation2415734 1 CMV promoter + RBS range2415734 1 1 654 annotation2415735 1 BBa_I712004 range2415735 1 1 654 BBa_K1366104_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaatatgcatcatcaccatcaccacatgaatgctctccaggaggacacaccccctggcccttctacagtcttccggccccctacaagctcacgacccctggagacacctcactgccgggagatcagaattggcatcgccggaattaccatcacactgagcctgtgcggatgtgccaacgctagggcacctactctgcgctccgcaaccgctgacaacagcgaatccacaggcttcaagaatgtgccagacctgcggaccgatcagcccaaacccccttctaagaaacgaagttgtgatccctcagagtacagagtcagtgaactgaaggaaagtctgattactactactccttcaagacctcgaaccgctaaacgacgaatccggctgtgagacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z