BBa_K1366108 1 C9 Apoptin and survivin siRNA production (C9) 2014-10-09T11:00:00Z 2015-05-08T01:10:08Z The apoptin DNA sequence was retrieved from NCBI with the accession number of NC_001427.1. The siRNA was designed by us. And the rest of the parts were from the registry. This construct will be used to characterize the transfection of two effectors of death (Survivin siRNA and apoptin) in mammalian cells mediated by bacteriophages. The survivin siRNA is expressed under an hTERT promoter, specific for cancerous cells lines. The apoptin is expressed under a CMV promoter because it is extremely specific, so it wouldn't harm the normal cells. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. false false _1743_ 0 16721 9 It's complicated false The construct structure is as follows: CMV // Apoptin // terminator // hTERT // siRNA // terminator false Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu annotation2415792 1 hTERT range2415792 1 1503 1962 annotation2415790 1 Apoptin range2415790 1 655 1020 annotation2415791 1 hGH polyadenylation range2415791 1 1021 1502 annotation2415793 1 survivin siRNA 1 range2415793 1 1963 2014 annotation2415789 1 hGH polyadenylation range2415789 1 2015 2496 annotation2415788 1 CMV + RBS range2415788 1 1 654 BBa_K1366108_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaatatgaacgcactccaggaggacacaccccctggacctagcacagtctttcggccccctacaagctcacgacccctggagacacctcactgccgggagatcagaattggcatcgccggaattaccatcacactgagcctgtgcggatgtgccaacgctagggcacctactctgcgctccgcaaccgctgacaacagcgaatccacaggcttcaagaatgtgccagacctgcggaccgatcagcccaaacccccttctaagaaacgaagttgtgatccctcagagtacagagtcagtgaactgaaggaatctctgatcactactactccttcacgacctcgaaccgctaagcgacgaattagactgtgagacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtcctttgtggcccctccctcgggttaccccacagcctaggccgattcgacctctctccgctggggccctcgctggcgtccctgcaccctgggagcgcgagcggcgcgcgggcggggaagcgcggcccagacccccgggtccgcccggagcagctgcgctgtcggggccaggccgggctcccagtggattcgcgggcacagacgcccaggaccgcgctccccacgtggcggagggactggggacccgggcacccgtcctgccccttcaccttccagctccgcctcctccgcgcggaccccgccccgtcccgacccctcccgggtccccggcccagccccctccgggccctcccagcccctccccttcctttccgcggccccgccctctcctcgcggcgcgagtttcaggcagcgctgcgtcctgctgcgcacgtgggaagccctggccccggccacccccgcgattggaccaccgcatctctacaatatttcaagagaatattgtagagatgcggtggtccgacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z