LOCUS       BBa_K1368000             939 bp    DNA     linear       17-May-2024
DEFINITION  pc1+lox71+TetR
ACCESSION   BBa_K1368000
VERSION     BBa_K1368000.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    58..91
                     /label=BBa_K886000
     misc_feature    104..107
                     /label=conserved
     RBS             100..111
                     /label=BBa_B0034
     CDS             121..737
                     /label=tetR
     misc_feature    738..771
                     /label=SsrA
     misc_feature    778..802
                     /label=Help:Barcodes
     CDS             118..777
                     /label=BBa_C0040
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
     misc_feature    100..939
                     /label=BBa_P0440
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagtac
       61 cgttcgtata gcatacatta tacgaagtta ttactagaga aagaggagaa atactagatg
      121 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
      181 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
      241 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
      301 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
      361 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
      421 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
      481 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
      541 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
      601 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
      661 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
      721 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      781 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttata
//