LOCUS       BBa_K1368002            1412 bp    DNA     linear       22-Nov-2024
DEFINITION  pter01+lox66+lacI
ACCESSION   BBa_K1368002
VERSION     BBa_K1368002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..96
                     /label=BBa_I718016
     misc_feature    109..112
                     /label=conserved
     RBS             105..116
                     /label=BBa_B0034
     misc_feature    1212..1250
                     /label=LVA
     CDS             123..1250
                     /label=lacI-LVA
     misc_feature    123..1250
                     /label=BBa_C0012
     misc_feature    1251..1275
                     /label=Help:Barcodes
     CDS             123..1250
                     /label=BBa_C0012
     stem_loop       1295..1338
                     /label=stem_loop
     misc_feature    1284..1363
                     /label=BBa_B0010
     terminator      1284..1363
                     /label=BBa_B0010
     stem_loop       1379..1398
                     /label=T7 TE
     misc_feature    1405..1405
                     /label=stop
     polyA_site      1399..1412
                     /label=polya
     misc_feature    1372..1412
                     /label=BBa_B0012
     terminator      1372..1412
                     /label=BBa_B0012
     misc_feature    105..1412
                     /label=BBa_P0412
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agataacttg gtatagcata cattatacga acggtatact agagaaagag gagaaatact
      121 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      181 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
      241 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
      301 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
      361 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
      421 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
      481 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
      541 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
      601 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
      661 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
      721 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
      781 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
      841 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
      901 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
      961 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     1021 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1081 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1201 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1261 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1321 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1381 ctcaccttcg ggtgggcctt tctgcgttta ta
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