BBa_K1368004
type: ComponentDefinition
persistent identity: BBa K1368004
display id: BBa_K1368004
version: 1
was generated by: iGEM to SBOL conversion
description: pluxL+lox66+aiiA
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:08Z
mutable provenance: to be finished
owned by: james
owned by: myers
mutable description: to be finished
discontinued: false
dominant: false
group u list: _1745_
m user id: 0
owner id: 23210
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: to be finished
star: false
creator: Yajing Zhang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0063
component: BBa_K516022
component: BBa_I718016
sequence annotation: BBa_R0063
sequence annotation: BBa_I718016
sequence annotation: BBa_K516022
sequence: https://synbiohub.org/public/igem/BBa_K1368004_sequence/1

BBa_R0063
type: Component
persistent identity: component2390105
display id: component2390105
version: 1
top level: BBa_K1368004
definition: lux pL
access: public

BBa_K516022
type: Component
persistent identity: component2390127
display id: component2390127
version: 1
top level: BBa_K1368004
definition: BBa_K516022
access: public

BBa_I718016
type: Component
persistent identity: component2390110
display id: component2390110
version: 1
top level: BBa_K1368004
definition: lox66
access: public

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2390105
display id: annotation2390105
version: 1
top level: BBa_K1368004
location: https://synbiohub.org/public/igem/BBa_K1368004/annotation2390105/range2390105/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K1368004/annotation2390105/range2390105/1
type: Range
persistent identity: range2390105
display id: range2390105
version: 1
top level: BBa_K1368004
direction: direction/forward
start: 1
end: 151
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation2390110
display id: annotation2390110
version: 1
top level: BBa_K1368004
location: https://synbiohub.org/public/igem/BBa_K1368004/annotation2390110/range2390110/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_K1368004/annotation2390110/range2390110/1
type: Range
persistent identity: range2390110
display id: range2390110
version: 1
top level: BBa_K1368004
direction: direction/reverse
start: 160
end: 193
orientation: inline

BBa_K516022
type: SequenceAnnotation
persistent identity: annotation2390127
display id: annotation2390127
version: 1
top level: BBa_K1368004
location: https://synbiohub.org/public/igem/BBa_K1368004/annotation2390127/range2390127/1
component: BBa_K516022

https://synbiohub.org/public/igem/BBa_K1368004/annotation2390127/range2390127/1
type: Range
persistent identity: range2390127
display id: range2390127
version: 1
top level: BBa_K1368004
direction: direction/reverse
start: 202
end: 1171
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pL)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
top level: lux pL
direction: direction/forward
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
top level: lux pL
direction: direction/forward
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
top level: lux pL
direction: direction/forward
start: 128
end: 128
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
top level: lux pL
direction: direction/forward
start: 130
end: 149
orientation: inline

lox66
type: ComponentDefinition
persistent identity: BBa I718016
display id: BBa_I718016
version: 1
was generated by: iGEM to SBOL conversion
description: lox66
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
owned by: james
owned by: myers
mutable description: lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No modidification was made on the lox66 sequence
star: true
creator: Eimad Shotar
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_I718016_sequence/1

BBa_K516022
type: ComponentDefinition
persistent identity: BBa K516022
display id: BBa_K516022
version: 1
was generated by: iGEM to SBOL conversion
description: AiiA protein generator TERM+ RBS+(B0032)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: AiiA protein generator TERM+ RBS+(B0032)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Susanna Zucca, Tommaso Goggia, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0015
component: BBa_C0060
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0060
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516022_sequence/1

BBa_B0015
type: Component
persistent identity: component2250570
display id: component2250570
version: 1
top level: BBa_K516022
definition: BBa_B0015
access: public

BBa_C0060
type: Component
persistent identity: component2250560
display id: component2250560
version: 1
top level: BBa_K516022
definition: aiiA
access: public

BBa_B0032
type: Component
persistent identity: component2250556
display id: component2250556
version: 1
top level: BBa_K516022
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250556
display id: annotation2250556
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
type: Range
persistent identity: range2250556
display id: range2250556
version: 1
top level: BBa_K516022
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation2250560
display id: annotation2250560
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
type: Range
persistent identity: range2250560
display id: range2250560
version: 1
top level: BBa_K516022
direction: direction/forward
start: 20
end: 808
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250570
display id: annotation2250570
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
type: Range
persistent identity: range2250570
display id: range2250570
version: 1
top level: BBa_K516022
direction: direction/reverse
start: 842
end: 970
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1368004_sequence/1
type: Sequence
persistent identity: BBa K1368004 sequence
display id: BBa_K1368004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagataacttggtatagcatacattatacgaacggtatactagagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0063_sequence/1
type: Sequence
persistent identity: BBa R0063 sequence
display id: BBa_R0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I718016_sequence/1
type: Sequence
persistent identity: BBa I718016 sequence
display id: BBa_I718016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttggtatagcatacattatacgaacggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516022_sequence/1
type: Sequence
persistent identity: BBa K516022 sequence
display id: BBa_K516022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z