LOCUS BBa_K1368006 1904 bp DNA linear 22-Nov-2024 DEFINITION pluxR+lox71+luxI+pc1+luxR ACCESSION BBa_K1368006 VERSION BBa_K1368006.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 64..97 /label=BBa_K886000 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 misc_feature 703..734 /label=LVA CDS 124..702 /label=luxI misc_feature 124..741 /label=BBa_C0061 misc_feature 742..766 /label=Help:Barcodes CDS 124..741 /label=BBa_C0061 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 terminator 775..903 /label=BBa_B0015 misc_feature 106..903 /label=BBa_K805016 misc_feature 1..903 /label=BBa_K1368005 promoter 926..931 /label=-35 promoter 936..952 /label=OR1 promoter 912..928 /label=OR2 promoter 949..954 /label=-10 misc_feature 912..960 /label=BBa_R1051 promoter 912..960 /label=BBa_R1051 misc_feature 973..976 /label=conserved RBS 969..980 /label=BBa_B0034 misc_feature 987..988 /label=prefix misc_feature 1160..1160 /label=T misc_feature 1478..1478 /label=A CDS 987..1736 /label=luxR misc_feature 987..1742 /label=BBa_C0062 misc_feature 1743..1767 /label=Help:Barcodes CDS 987..1742 /label=BBa_C0062 stem_loop 1787..1830 /label=stem_loop misc_feature 1776..1855 /label=BBa_B0010 terminator 1776..1855 /label=BBa_B0010 stem_loop 1871..1890 /label=T7 TE misc_feature 1897..1897 /label=stop polyA_site 1891..1904 /label=polya misc_feature 1864..1904 /label=BBa_B0012 terminator 1864..1904 /label=BBa_B0012 misc_feature 969..1904 /label=BBa_I0462 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagtaccgtt cgtatagcat acattatacg aagttattac tagagaaaga ggagaaatac 121 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa 181 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta 241 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt 301 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg 361 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata 421 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt 481 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca 541 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct 601 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg 661 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtaacaccgt gcgtgttgac aattttacct ctggcggtga tactggttgc 961 tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac acatacagaa 1021 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata 1081 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg 1141 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg 1201 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa 1261 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag 1321 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg 1381 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt 1441 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa 1501 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag 1561 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc 1621 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc 1681 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat 1741 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 1801 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1861 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //