LOCUS       BBa_K1368008            1315 bp    DNA     linear       15-May-2024
DEFINITION  mcherry generator driven by HSL under the post transcriptional 
            control
ACCESSION   BBa_K1368008
VERSION     BBa_K1368008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    64..157
                     /label=BBa_J01008
     stem_loop       177..220
                     /label=stem_loop
     misc_feature    166..245
                     /label=BBa_B0010
     terminator      166..245
                     /label=BBa_B0010
     stem_loop       261..280
                     /label=T7 TE
     misc_feature    287..287
                     /label=stop
     polyA_site      281..294
                     /label=polya
     misc_feature    254..294
                     /label=BBa_B0012
     terminator      254..294
                     /label=BBa_B0012
     misc_feature    64..294
                     /label=BBa_I714075
     misc_feature    303..322
                     /label=LuxR/HSL
     promoter        322..327
                     /label=-35
     promoter        344..349
                     /label=-10
     misc_feature    355..355
                     /label=start
     misc_feature    303..357
                     /label=BBa_R0062
     promoter        303..357
                     /label=BBa_R0062
     misc_feature    366..405
                     /label=BBa_J01010
     RBS             414..425
                     /label=B0034
     CDS             432..1178
                     /label=mCherry-LVA
     misc_feature    432..434
                     /label=Start
     misc_feature    1143..1172
                     /label=ssrA degradation tag: LVA
     misc_feature    1173..1175
                     /label=Stop
     misc_feature    1176..1178
                     /label=Stop
     misc_feature    414..1178
                     /label=BBa_K773003
     stem_loop       1198..1241
                     /label=stem_loop
     misc_feature    1187..1266
                     /label=BBa_B0010
     terminator      1187..1266
                     /label=BBa_B0010
     stem_loop       1282..1301
                     /label=T7 TE
     misc_feature    1308..1308
                     /label=stop
     polyA_site      1302..1315
                     /label=polya
     misc_feature    1275..1315
                     /label=BBa_B0012
     terminator      1275..1315
                     /label=BBa_B0012
     terminator      1187..1315
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagacccaaa tccaggaggt gaatctagta ggtggttaat gaaaattaac ttacttacta
      121 gaaatatctc taaaaagcca gattattaat ccggctttac tagagccagg catcaaataa
      181 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      241 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      301 agacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac
      361 tagagaacta gaatcacctc ttggatttgg gtattaaaga ggagatacta gagaaagagg
      421 agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      481 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      541 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      601 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      661 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      721 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      781 ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      841 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      901 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      961 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     1021 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     1081 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     1141 cagcaaacga cgaaaactac gctctagtag cttaataata ctagagccag gcatcaaata
     1201 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1261 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//