##gff-version 3
##sequence-region BBa_K1368008 1 1315
BBa_K1368008	.	engineered_region	64	294	.	+	0	ID=BBa_I714075;Name=BBa_I714075
BBa_K1368008	.	mature_transcript_region	64	157	.	+	0	ID=BBa_J01008;Name=BBa_J01008;Parent=BBa_I714075
BBa_K1368008	.	terminator	254	294	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I714075
BBa_K1368008	.	polyA_site	281	294	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1368008	.	stem_loop	261	280	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1368008	.	engineered_region	254	294	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1368008	.	stop_codon	287	287	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1368008	.	terminator	166	245	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I714075
BBa_K1368008	.	stem_loop	177	220	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1368008	.	engineered_region	166	245	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1368008	.	promoter	303	357	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1368008	.	start_codon	355	355	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1368008	.	promoter	322	327	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1368008	.	promoter	344	349	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1368008	.	engineered_region	303	357	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1368008	.	non_covalent_binding_site	303	322	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1368008	.	mature_transcript_region	366	405	.	+	0	ID=BBa_J01010;Name=BBa_J01010
BBa_K1368008	.	terminator	1187	1315	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1368008	.	terminator	1275	1315	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1368008	.	polyA_site	1302	1315	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1368008	.	stem_loop	1282	1301	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1368008	.	engineered_region	1275	1315	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1368008	.	stop_codon	1308	1308	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1368008	.	terminator	1187	1266	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1368008	.	stem_loop	1198	1241	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1368008	.	engineered_region	1187	1266	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1368008	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1368008	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1368008	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1368008	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1368008	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1368008	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1368008	.	engineered_region	414	1178	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1368008	.	start_codon	432	434	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1368008	.	stop_codon	1173	1175	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1368008	.	stop_codon	1176	1178	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1368008	.	tag	1143	1172	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1368008	.	CDS	432	1178	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1368008	.	ribosome_entry_site	414	425	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
>BBa_K1368008
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagacccaaatccaggagg
tgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttt
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgt
acaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaactagaatcacctcttggatttgggtatt
aaagaggagatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaagg
agttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccg
cccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcc
cctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccg
agggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcagga
cggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatg
ggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctga
aggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaa
cgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgc
cactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata