LOCUS BBa_K1368009 1585 bp DNA linear 25-Nov-2024 DEFINITION Pluxr+taRNA+T+Pluxr+CrRNA+cre ACCESSION BBa_K1368009 VERSION BBa_K1368009.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 64..157 /label=BBa_J01008 stem_loop 177..220 /label=stem_loop misc_feature 166..245 /label=BBa_B0010 terminator 166..245 /label=BBa_B0010 stem_loop 261..280 /label=T7 TE misc_feature 287..287 /label=stop polyA_site 281..294 /label=polya misc_feature 254..294 /label=BBa_B0012 terminator 254..294 /label=BBa_B0012 misc_feature 64..294 /label=BBa_I714075 misc_feature 303..322 /label=LuxR/HSL promoter 322..327 /label=-35 promoter 344..349 /label=-10 misc_feature 355..355 /label=start misc_feature 303..357 /label=BBa_R0062 promoter 303..357 /label=BBa_R0062 misc_feature 366..405 /label=BBa_J01010 CDS 412..1448 /label=BBa_J61047 stem_loop 1468..1511 /label=stem_loop misc_feature 1457..1536 /label=BBa_B0010 terminator 1457..1536 /label=BBa_B0010 stem_loop 1552..1571 /label=T7 TE misc_feature 1578..1578 /label=stop polyA_site 1572..1585 /label=polya misc_feature 1545..1585 /label=BBa_B0012 terminator 1545..1585 /label=BBa_B0012 terminator 1457..1585 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagacccaaa tccaggaggt gaatctagta ggtggttaat gaaaattaac ttacttacta 121 gaaatatctc taaaaagcca gattattaat ccggctttac tagagccagg catcaaataa 181 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 241 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 301 agacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac 361 tagagaacta gaatcacctc ttggatttgg gtattaaaga ggagatacta gatgtccaat 421 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt 481 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa 541 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg 601 tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg 661 gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg 721 ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa 781 aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac 841 caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt 901 ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa 961 gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg 1021 gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga 1081 tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga 1141 aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg 1201 atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac 1261 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga 1321 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac 1381 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat 1441 taagaattta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1501 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1561 tcgggtgggc ctttctgcgt ttata //