LOCUS       BBa_K1368012            1875 bp    DNA     linear       11-May-2024
DEFINITION  pc1+mCherry+plac+ECFP
ACCESSION   BBa_K1368012
VERSION     BBa_K1368012.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..786
                     /label=mCherry
     misc_feature    427..427
                     /label=C->T (removing PstI site)
     misc_feature    76..789
                     /label=BBa_J06504
     misc_feature    58..789
                     /label=BBa_J06602
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
     terminator      798..926
                     /label=BBa_B0015
     misc_feature    937..953
                     /label=lac O1
     promoter        954..959
                     /label=-35
     misc_feature    960..976
                     /label=lac O1
     promoter        977..982
                     /label=-10
     misc_feature    935..988
                     /label=BBa_R0011
     promoter        935..988
                     /label=BBa_R0011
     misc_feature    1002..1005
                     /label=conserved
     RBS             998..1009
                     /label=BBa_B0034
     CDS             1016..1738
                     /label=BBa_E0020
     stem_loop       1758..1801
                     /label=stem_loop
     misc_feature    1747..1826
                     /label=BBa_B0010
     terminator      1747..1826
                     /label=BBa_B0010
     stem_loop       1842..1861
                     /label=T7 TE
     misc_feature    1868..1868
                     /label=stop
     polyA_site      1862..1875
                     /label=polya
     misc_feature    1835..1875
                     /label=BBa_B0012
     terminator      1835..1875
                     /label=BBa_B0012
     misc_feature    998..1875
                     /label=BBa_E0420
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa caattgacat
      961 tgtgagcgga taacaagata ctgagcacat actagagaaa gaggagaaat actagatggt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     1501 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata
     1741 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1801 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1861 ctttctgcgt ttata
//