BBa_K1369001
1
BBa_K1369001
Repressor-Repressor promoter (LacI and TetR operator sites)
2014-08-17T11:00:00Z
2015-05-08T01:10:08Z
This part is taken from the paper "Programming gene expression with combinatorial promoters," by Robert Sidney Cox III et al.
This is a repressor-repressor (RR) combinatorial promoter which consists of a TetR operator site within the -35 and -10 RNA polymerase boxes and a LacI operator site downstream of the -10 RNA polymerase box.
false
false
_1746_
0
20314
9
Not in stock
false
To test the logic of this RR promoter, we assembled constructs containing the promoter, a bicistronic RBS (part BBa_J119024), and GFP, in that order, downstream of the promoter.
false
annotation2381240
1
-35 RNA polymerase box
range2381240
1
39
43
annotation2381239
1
LacI operator site
range2381239
1
1
42
annotation2381241
1
-10 RNA polymerase box
range2381241
1
62
66
annotation2381242
1
TetR operator site
range2381242
1
45
66
BBa_K1369001_sequence
1
tacaacgtctgttagctgcaattgtgagcggataacaattgacaaataactctatcaatgatagagttcgttatcaccgccaggggtaaggcgttacccaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z