LOCUS       BBa_K1369004            2019 bp    DNA     linear       23-Dec-2024
DEFINITION  AgrA+SH3 peptide; P2 promoter+GFP
ACCESSION   BBa_K1369004
VERSION     BBa_K1369004.1  
FEATURES             Location/Qualifiers
     RBS             286..317
                     /label=RBS
     CDS             318..941
                     /label=AgrA
     misc_feature    942..977
                     /label=GS linker
     misc_feature    978..1010
                     /label=SH3 pep
     misc_feature    1011..1019
                     /label=stop codon
     misc_feature    1026..1154
                     /label=double terminator
     promoter        1167..1262
                     /label=P2 promoter
     RBS             1263..1302
                     /label=RBS
     misc_feature    1303..2019
                     /label=sfGFP
     promoter        1..285
                     /label=pBAD
ORIGIN
        1 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
       61 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
      121 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
      181 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
      241 tcttacctga cgctttttat cgcaactctc tactgtttct ccatacccgc tagcggtacc
      301 tcacgcgata cagatctatg gaaatcgcac tggctacgga caatccatat gaggttctgg
      361 aacaggctaa aaacatgaac gacatcggct gctatttcct ggatattcag ctgagcaccg
      421 acatcaacgg tatcaaactg ggctctgaaa tccgtaaaca tgatccggtt ggtaacatta
      481 ttttcgtgac ttctcattct gagctgacct atctgacgtt cgtatacaag gttgcagcca
      541 tggatttcat ctttaaagac gatccggcag agctgcgtac gcgcatcatc gactgtctgg
      601 aaactgcgca cacgcgcctg caactgctgt ccaaagataa ctccgtggaa accattgaac
      661 tgaaacgtgg cagcaacagc gtttacgttc agtacgatga tatcatgttc ttcgaaagct
      721 ccactaagtc ccaccgtctg atcgcgcatc tggataatcg tcagatcgag ttctatggca
      781 acctgaaaga gctgagccag ctggacgatc gcttcttccg ttgtcataac tccttcgttg
      841 ttaaccgtca caacatcgaa tccatcgact ccaaagagcg tattgtatac ttcaagaaca
      901 aagaacactg ttatgcgtct gtacgcaatg tcaagaaaat cggatctggt agtggcagcg
      961 gcagcggtag tggatctccg ccgcctgctc tgccgccaaa aagaaggcgg ggatcctaac
     1021 tcgagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1081 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1141 tttctgcgtt tataccgcgg cctaggatta aatacaaatt acatttaaca gttaagtatt
     1201 tatttcctac agttaggcaa tataatgata aaagattgta ctaaatcgta taatgacagt
     1261 gagagctcat acattcgtaa gaaacgggcg caggaggatt agatgagtaa aggagaagaa
     1321 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1381 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     1441 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttgacttat
     1501 ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt tttcaagagt
     1561 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactat
     1621 aagacacgtg ctgaagtcaa gtttgaaggt gatacacttg ttaatagaat cgagttaaaa
     1681 ggtattgatt ttaaagaaga tggaaacatt cttggacaca agttggaata caactataac
     1741 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     1801 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     1861 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1921 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     1981 gccgggatta cacatggcat ggatgaacta tacaaatag
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