BBa_K1369004
1
BBa_K1369004
AgrA+SH3 peptide; P2 promoter+GFP
2014-09-03T11:00:00Z
2015-05-08T01:10:08Z
The AgrA response regulator and P2 promoter are endogenous to Staphylococcus aureus, which are involved in the AgrBDCA quorum sensing system.
This sequence is composed of the AgrA response regulator with an SH3 peptide attached to the N-terminus. Downstream of the AgrA coding sequence is a GFP reporter gene which is regulated by the P2 promoter.
false
false
_1746_
0
20314
9
Not in stock
false
A double terminator sequence separates the AgrA sequence from the P2 promoter sequence.
false
Andrew Hou
annotation2393376
1
pBAD
range2393376
1
1
285
annotation2393371
1
stop codon
range2393371
1
1011
1019
annotation2393367
1
RBS
range2393367
1
286
317
annotation2393370
1
SH3 pep
range2393370
1
978
1010
annotation2393373
1
P2 promoter
range2393373
1
1167
1262
annotation2393369
1
GS linker
range2393369
1
942
977
annotation2393372
1
double terminator
range2393372
1
1026
1154
annotation2393375
1
sfGFP
range2393375
1
1303
2019
annotation2393368
1
AgrA
range2393368
1
318
941
annotation2393374
1
RBS
range2393374
1
1263
1302
BBa_K1369004_sequence
1
agaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccgctagcggtacctcacgcgatacagatctatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcggatctggtagtggcagcggcagcggtagtggatctccgccgcctgctctgccgccaaaaagaaggcggggatcctaactcgagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccgcggcctaggattaaatacaaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtgagagctcatacattcgtaagaaacgggcgcaggaggattagatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactataagacacgtgctgaagtcaagtttgaaggtgatacacttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgccgggattacacatggcatggatgaactatacaaatag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z