LOCUS BBa_K1369007 9200 bp DNA linear 25-Nov-2024 DEFINITION pBAD FsrA-SH3pep, pFsrA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations wil ACCESSION BBa_K1369007 VERSION BBa_K1369007.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcggaag ctgaattcac tagtctatgc tactccatcg agccgtcaat tgtctgattc 61 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg 121 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg 181 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc 241 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg 301 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata 361 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta 421 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca 481 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt 541 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta 601 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa 661 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct 721 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc 781 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg 841 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca 901 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc 961 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc 1021 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa 1081 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga 1141 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 1201 ataagattag cggatcttac ctgacgcttt ttatcgcaac tctctactgt ttctccatac 1261 ccgctagcgg tacctcacgc gatacagatc tatgagtgaa caaatggcta tttatatatt 1321 agaagaccaa attattcaag caaaagcgtt agaagcattg gtaacaaata tcctacattc 1381 aagaaatatt tataatgaaa caatccattt attttcacgt tcagatgaat tattacaagt 1441 ggcacaccag gacgcccaac taaatatctt tttcctagat attcaaatga acaatcatat 1501 ccaggcagga tttgaggttg ctaaagaaat tagaaaaaca gacagtgaaa gtctgattgt 1561 atttatttct actcacacag aattagtttt aacttcctac aaatatatgg tttcagctct 1621 tcaatttatt caaaagaacg tggattttct agatttccga aaagaagttg aaacatgtgt 1681 cgacgcatat attcaacaaa aagaaaatat aaagacaaaa agtgaatata ttatcattaa 1741 cttaaaagcg tcttctataa aaatggatat taatgatatt tatttctttc aaacggaata 1801 cgatcatcgt gtgtcaatgg ttggtaaaaa ttttaaaaga gaattttatg gaacgttatc 1861 aaaaattgaa caactgcatc cagatttgat tagagtccat caatcaatca tcatcaataa 1921 aaaatatgcg tcaaaattaa attataaaac gcatctttta actatgagag acggaacgga 1981 agtaccagtc tcaagaagat attatactca ggtcaaggcg ctatttctta cttagggatc 2041 tggtagtggc agcggcagcg gtagtggatc tccgccgcct gctctgccgc caaaaagaag 2101 gcggggatcc taactcgagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2161 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2221 ccttcgggtg ggcctttctg cgtttatacc gcggcctagg cttacttagg gagggataag 2281 agctcataca ttcgtaagaa acgggcgcag gaggattaga tgagtaaagg agaagaactt 2341 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 2401 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 2461 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt gacttatggt 2521 gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc 2581 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactataag 2641 acacgtgctg aagtcaagtt tgaaggtgat acacttgtta atagaatcga gttaaaaggt 2701 attgatttta aagaagatgg aaacattctt ggacacaagt tggaatacaa ctataactca 2761 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 2821 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 2881 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 2941 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgcc 3001 gggattacac atggcatgga tgaactatac aaatagtgag aagcttgggc ccgaacaaaa 3061 actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt 3121 ttaaacggtc tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag 3181 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg 3241 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt 3301 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca 3361 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaactaat ttctagacca 3421 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt 3481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 3541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 3601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 3661 cggggagagg cggtttgcgt attgggcgca tgcataaaaa ctgttgtaat tcattaagca 3721 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca 3781 gcaccttgtc gccttgcgta taatatttgc ccatgggggt gggcgaagaa ctccagcatg 3841 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac 3901 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg 3961 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg 4021 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt 4081 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg 4141 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat 4201 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct 4261 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct 4321 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt 4381 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga 4441 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc 4501 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa 4561 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac 4621 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg 4681 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc 4741 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc 4801 ctgtctcttg atcagatttt gatcccctgc gccatcagat ccttggcggc aagaaagcca 4861 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg 4921 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag 4981 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat 5041 tcatcccagg tggcactttt cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc 5101 ctgtttctgg cgctggactt cccgctgttc cgtcagcagc ttttcgccca cggccttgat 5161 gatcgcggcg gccttggcct gcatatcccg attcaacggc cccagggcgt ccagaacggg 5221 cttcaggcgc tcccgaaggt ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat 5281 ctcacgcgct cctgcggcgg cctgtagggc aggctcatac ccctgccgaa ccgcttttgt 5341 cagccggtcg gccacggctt ccggcgtctc aacgcgcttt gagattccca gcttttcggc 5401 caatccctgc ggtgcatagg cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc 5461 cactggtggc cgctccaggg cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct 5521 gccctcgatg ccccgttgca gccctagatc ggccacagcg gccgcaaacg tggtctggtc 5581 gcgggtcatc tgcgctttgt tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt 5641 cagcggcacc acgaacgcgg tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt 5701 cacgatgcga tccgccccgt acttgtccgc cagccacttg tgcgccttct cgaagaacgc 5761 cgcctgctgt tcttggctgg ccgacttcca ccattccggg ctggccgtca tgacgtactc 5821 gaccgccaac acagcgtcct tgcgccgctt ctctggcagc aactcgcgca gtcggcccat 5881 cgcttcatcg gtgctgctgg ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc 5941 gttgggcgtc tcgcgctcgc ggtaggcgtg cttgagactg gccgccacgt tgcccatttt 6001 cgccagcttc ttgcatcgca tgatcgcgta tgccgccatg cctgcccctc ccttttggtg 6061 tccaaccggc tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg 6121 agtatactca ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc 6181 tacgggcttg ctctccgggc ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg 6241 gatatgtgga cgatggccgc gagcggccac cggctggctc gcttcgctcg gcccgtggac 6301 aaccctgctg gacaagctga tggacaggct gcgcctgccc acgagcttga ccacagggat 6361 tgcccaccgg ctacccagcc ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg 6421 gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc tgcggcctgc 6481 gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg 6541 cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc 6601 gaaggcgaag cccgcccgcc tgccccccga gcctcacggc ggcgagtgcg ggggttccaa 6661 gggggcagcg ccaccttggg caaggccgaa ggccgcgcag tcgatcaaca agccccggag 6721 gggccacttt ttgccggagg gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg 6781 cccttctttg ggcaccaaag aactagatat agggcgaaat gcgaaagact taaaaatcaa 6841 caacttaaaa aaggggggta cgcaacagct cattgcggca ccccccgcaa tagctcattg 6901 cgtaggttaa agaaaatctg taattgactg ccacttttac gcaacgcata attgttgtcg 6961 cgctgccgaa aagttgcagc tgattgcgca tggtgccgca accgtgcggc accctaccgc 7021 atggagataa gcatggccac gcagtccaga gaaatcggca ttcaagccaa gaacaagccc 7081 ggtcactggg tgcaaacgga acgcaaagcg catgaggcgt gggccgggct tattgcgagg 7141 aaacccacgg cggcaatgct gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc 7201 gtggtggtca gccagaagac actttccaag ctcatcggac gttctttgcg gacggtccaa 7261 tacgcagtca aggacttggt ggccgagcgc tggatctccg tcgtgaagct caacggcccc 7321 ggcaccgtgt cggcctacgt ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag 7381 ttgcgcctgt cggtgttcag tgccgccgtg gtggttgatc acgacgacca ggacgaatcg 7441 ctgttggggc atggcgacct gcgccgcatc ccgaccctgt atccgggcga gcagcaacta 7501 ccgaccggcc ccggcgagga gccgcccagc cagcccggca ttccgggcat ggaaccagac 7561 ctgccagcct tgaccgaaac ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg 7621 cccgatgagc cgtgttttct ggacgatggc gagccgttgg agccgccgac acgggtcacg 7681 ctgccgcgcc ggtagcactt gggttgcgca gcaacccgta agtgcgctgt tccagactat 7741 cggctgtagc cgcctcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc 7801 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc 7861 gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg 7921 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg 7981 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct 8041 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac 8101 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc 8161 cccgccctgc cactcatcgc agtcggccta ttggttaaaa aatgagctga tttaacaaaa 8221 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg 8281 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 8341 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 8401 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctg 8461 gggccgcaat tcccaattcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 8521 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg 8581 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat 8641 aaactgccag gaattaattc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 8701 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 8761 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca 8821 taaactgcca ggaattaatt ccaggcatca aataaaacga aaggctcagt cgaaagactg 8881 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 8941 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc 9001 ataaactgcc aggaattaat tccaggcatc aaataaaacg aaaggctcag tcgaaagact 9061 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc 9121 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc 9181 cataaactgc caggaattgg //