BBa_K1369008 1 BBa_K1369008 pCon FsrC, constituatively expressed non-inhibited HK with 4 SH3 domains 2014-10-08T11:00:00Z 2015-05-08T01:10:08Z Enterococcus faecalis This plasmid contains FsrC, which is a histidine kinase used in E. coli. It has 4 SH3 domains, which lets the scaffold protein attach so that we can test the two component system without a ligand. In our project, we were designing a two-component system, with a histidine kinase and response regulator. If there is a change in the environment that the histidine kinase sense, the response regulator will act in response to it and express GFP. false false _1746_ 0 20316 9 Not in stock false It contains 4 SH3 domains false Monique Alkiewicz BBa_K1369008_sequence 1 gaattcactagtttgacagttagctcagtcctaggaattatgctagcggtacctcccggtgccctctaccagggccaagagatctatgattttgtcgttattagctactaacgttttgctggcatctagctttatcgtttttgtctttttacgagtaacattgatcaagattgaatgtaaaataccgttactttcattgcttattgtcattaatctttgttcgtttgcggcattaatgttgggctactcttggttgatttatgcgctgacagtcgttgtttttacaggatttttactcattcacaaaaagaggttctcaatttttaaagcgatatttttatccgtttttacattgcttatggtttcgtttatcaattacacggagcaaacgattttaagtgttttttttcaacagatttatcaaaataaattattatggattgcctcaaatgttcttctgttgcttataaatatctggattgctttaaaaattcccaatagtgtttttttaagattaaatcgtgtgttagaaaatagccgaattttttttggttgtttacttttattgttgattctgttgttactttttgtgtttttgatttcgccagagatttcacctgactttatgcgaggatttgtcacggtaaatagttctaaattggagttattaataagtgtaggtttatttttaattctgattggcttagtcattgaagcttatttggaagaacaacgtatcaacactcaattattgaataatttaacgatttatactgaaaaaatagaatccattaacgaagagctagcgatgtttcgtcatgattataaaaatttattgtatagtttacaaattgctatttcatacgaagatattctggaaatcaaaagaatttatgaagaaacgattgcaccaaccaaaaaaattattgataatgaagaatttgaacttatgaagttaaatcgtttgaaaaatatggaacttaaggcactcataagtatgaaaattaataccgcaaagcaagcaaaactaaaagtgattgttgatgtgccagaagtatttattcttgatacatcaatcgacttagtggttgtgattcgtttgttggctattctattagataacgcgattgaaaacagtgcaaaatcagaattgaaaatgttcgctatctcaattttcaataaaaatgaaacacaagagtttgtgataacaaatagtgtccaagccgaatttgattttagggttatgaagaaaaccaaatttagttctaaaagtaacccagaaaagcacggttggggattgttatatgtaaaagaaattgttgatttttcagatcaatttgacttgcaaacgtccttcaatgaaggatcggttactcaacatttaattattgaaaaaaatcataacagtaaaaaagttgttaacgaatgaggatctggtagtggcagcggcagcggtagtggatctggtagtggcagcggcagcggtagtggatctatggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatggatctggtagtggcagcggcagcggtagtggatctatggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatggatctggtagtggcagcggcagcggtagtggatctatggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatggatctggtagtggcagcggcagcggtagtggatctatggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatggatcctgactcgagtaatgagaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaactaatttctagactgcagttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgatatcgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcctcggtgagaatccaagcctcgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtatttattcggcgcaaagtgcgtcgggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcggtagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcttatcatcgataagcttccgatggcgcgccgagaggctttacactttatgcttccggct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z