LOCUS BBa_K1369010 9190 bp DNA linear 22-Nov-2024 DEFINITION pBAD LamA-L-SH3pep, pLamA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations w ACCESSION BBa_K1369010 VERSION BBa_K1369010.1 FEATURES Location/Qualifiers ORIGIN 1 ggatcggaag ctgaattcac tagtctatgc tactccatcg agccgtcaat tgtctgattc 61 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg 121 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg 181 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc 241 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg 301 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata 361 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta 421 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca 481 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt 541 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta 601 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa 661 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct 721 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc 781 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg 841 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca 901 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc 961 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc 1021 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa 1081 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga 1141 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 1201 ataagattag cggatcttac ctgacgcttt ttatcgcaac tctctactgt ttctccatac 1261 ccgctagcgg tacctcacgc gatacagatc tttatgactt tttagcaagc catttagcta 1321 gataacgatt agcttgtgca gaacaaagca ctttttcacc atttgtcatg gttaatcgtc 1381 gcaagtgctt gtccaatgac ttaatgcgtt tcaagttaac aacatatgac ttatgacatc 1441 tatagaaatt aggactcaaa tcaggaacat cctttaacag tccatagtat tctaattcac 1501 catcatccaa atggactacg attttatggg gactggttga actttcaaaa aatataatgc 1561 ttttaacatt aattgatctg accgtctcag caatttttat ttgaacatag tcgtcttggt 1621 cattttcatc ggtttgaaaa cgatcttgcg ccacgtgtaa aaccttgcta aatcgctcat 1681 acaaggaatc tggaaaatcc ttaagaataa aatctaatgc ctcaacttga tattgaaaca 1741 ccatcaaaga taactccgtg tgtgtcgtga taaaaataat cttgctgcgc cggcccatgt 1801 cacgaatctg gctagctagt tgaataccat taacatctga attaagggca atatctaaca 1861 catagattgc cttttgaggc tgctgtgcat taaccttgtc taacaattcg tggggatctt 1921 ggcatgctaa acagatactc atgtctagct gttgatcgac gattacctta gtaatgattt 1981 gccgtatttg ttgtaattgt ttcggatcat cttcgcaaat atagacacta atcatggatc 2041 tggtagtggc agcggcagcg gtagtggatc tccgccgcct gctctgccgc caaaaagaag 2101 gcggggatcc taactcgagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2161 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2221 ccttcgggtg ggcctttctg cgtttatacc gcggcctagg tctttaaatg agctcataca 2281 ttcgtaagaa acgggcgcag gaggattaga tgagtaaagg agaagaactt ttcactggag 2341 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 2401 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 2461 gaaaactacc tgttccatgg ccaacacttg tcactacttt gacttatggt gttcaatgct 2521 tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag 2581 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactataag acacgtgctg 2641 aagtcaagtt tgaaggtgat acacttgtta atagaatcga gttaaaaggt attgatttta 2701 aagaagatgg aaacattctt ggacacaagt tggaatacaa ctataactca cacaatgtat 2761 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 2821 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 2881 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 2941 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgcc gggattacac 3001 atggcatgga tgaactatac aaatagtgag aagcttgggc ccgaacaaaa actcatctca 3061 gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc 3121 tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 3181 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3241 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3301 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3361 tgggcctttc gttttatctg ttgtttgtcg gtgaactaat ttctagacca gcttttgttc 3421 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg 3481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 3541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3661 cggtttgcgt attgggcgca tgcataaaaa ctgttgtaat tcattaagca ttctgccgac 3721 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 3781 gccttgcgta taatatttgc ccatgggggt gggcgaagaa ctccagcatg agatccccgc 3841 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga 3901 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt 3961 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg 4021 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag 4081 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag 4141 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca 4201 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc 4261 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag 4321 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg 4381 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt 4441 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag 4501 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt 4561 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc 4621 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga 4681 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg 4741 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg 4801 atcagatttt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac 4861 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc 4921 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt 4981 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatcccagg 5041 tggcactttt cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc ctgtttctgg 5101 cgctggactt cccgctgttc cgtcagcagc ttttcgccca cggccttgat gatcgcggcg 5161 gccttggcct gcatatcccg attcaacggc cccagggcgt ccagaacggg cttcaggcgc 5221 tcccgaaggt ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct 5281 cctgcggcgg cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg 5341 gccacggctt ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc 5401 ggtgcatagg cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc 5461 cgctccaggg cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg 5521 ccccgttgca gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc 5581 tgcgctttgt tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc 5641 acgaacgcgg tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga 5701 tccgccccgt acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt 5761 tcttggctgg ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac 5821 acagcgtcct tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg 5881 gtgctgctgg ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc 5941 tcgcgctcgc ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc 6001 ttgcatcgca tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc 6061 tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca 6121 ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg 6181 ctctccgggc ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga 6241 cgatggccgc gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg 6301 gacaagctga tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg 6361 ctacccagcc ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc 6421 atcaattttt ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct 6481 tgccggttgg acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct 6541 tggccttgac gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag 6601 cccgcccgcc tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg 6661 ccaccttggg caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt 6721 ttgccggagg gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg 6781 ggcaccaaag aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa 6841 aaggggggta cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa 6901 agaaaatctg taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa 6961 aagttgcagc tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa 7021 gcatggccac gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg 7081 tgcaaacgga acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg 7141 cggcaatgct gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca 7201 gccagaagac actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca 7261 aggacttggt ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt 7321 cggcctacgt ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt 7381 cggtgttcag tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc 7441 atggcgacct gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc 7501 ccggcgagga gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct 7561 tgaccgaaac ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc 7621 cgtgttttct ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc 7681 ggtagcactt gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc 7741 cgcctcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg 7801 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca 7861 ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct 7921 gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata 7981 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga 8041 ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc 8101 gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc 8161 cactcatcgc agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 8221 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt 8281 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 8341 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 8401 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat 8461 tcccaattcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 8521 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt 8581 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag 8641 gaattaattc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 8701 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt 8761 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca 8821 ggaattaatt ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 8881 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat 8941 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc 9001 aggaattaat tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 9061 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga 9121 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc 9181 caggaattgg //