BBa_K1369013
1
BBa_K1369013
lamBD_3xFLAG_N
2014-10-16T11:00:00Z
2015-05-08T01:10:08Z
Lactobacillus Plantarum
Same as part BBa_K1369012 (lamBD genes from Lactobacillus plantarum) but a 3xFLAG domain has been inserted at the N-terminus (5' end) of the lamD gene. This 3xFLAG sequence serves as a marker for antibody-binding in Western blotting and purification columns. Part available upon request from Caltech 2014 iGEM team in a custom backbone.
false
false
_1746_
0
20035
9
Not in stock
false
Inserted 3xFLAG at N-terminus (5' end) of lamD, which is the prepeptide of the signaling ligand.
Assembled via Gibson assembly. Not biobricked.
false
Galen Gao
BBa_K1369013_sequence
1
atggaaaagccagaacaaaaactattgctttataaattaagcgaccggttattcgctgcgattcaaaagaatcttcaactagaaagacggcaggcattactagttaaactaggcattgatactgtattaaatgttattcctaagctgataattactattatcttagcgttgctgctacacgagttggtcccagtgttagtttttatgggtagttttttagttttgcgtggctttgcctatggacgtcacttggaatcagatctgttatgtacgatattaacagcagtgacgttcgtgggtgtgccatatttaatacagtttactgatggcattccagaactttttagatttatattgtgtttgctgttaactgtacctattgggatgttttcaccggctgttacaagaaagaatccaattaaatcgcagtcattaaaaagggctttaaagcataaagcaatcattacgtcacttgtttttagttttttacaatttttagttagtaataatctgggaacaataattgttgtatcgctgttattagttttcacattaatagttccattgaaaggagggaagtctgatgaagcagaaaatgtatgaatggactacaaggaccatgacggtgattacaaggatcatgacatcgactacaaggatgacgatgacaaggactacaaggaccatgacggtgattacaaggatcatgacatcgactacaaggatgacgatgacaagaagcagaaaatgtatgaagctattgcacatctttttaaatatgttggcgcgaaacaattagtaatgtgctgtgtaggaatttggtttgaaactaaaattcctgacgaactacgtaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z